目前的理解强调白血病细胞与其环境之间复杂的相互作用。血小板衍生的微粒在促进细胞间通讯方面发挥着至关重要的作用，并有助于复杂的癌症病理学景观。本研究旨在探讨血小板衍生微粒对慢性粒细胞白血病细胞系中细胞增殖、凋亡以及关键基因表达的影响，包括 P53、P21、Cyclin D1、Bax 和 Bcl-2。 (K562).通过以不同速度离心获得血小板衍生的微粒，并使用BCA测定对其浓度进行定量。为了确定 PMP 的大小和免疫表型特征，采用了 DLS 技术和流式细胞术。使用MTT测定和血细胞计数器评估细胞增殖，并通过DNA含量评估进行细胞周期分析。利用实时 PCR 对 Bax、Bcl-2、Cyclin D1、P53 和 P21 的基因表达进行分析。采用流式细胞术检测细胞凋亡。研究结果表明，血小板衍生的微粒能够减少 K562 细胞系的增殖，同时不会对细胞凋亡和细胞周期进程产生影响。通过实时 PCR 分析表明 P53、P21 和 Bcl-2 基因表达上调，同时 Bax 和 Cyclin D1 基因表达下调。这项研究揭示了慢性粒细胞白血病与其微环境之间的复杂关系，特别是血小板衍生微粒的参与。该研究强调了血小板衍生微粒影响细胞行为和基因表达的潜力，让人们更深入地了解它们在 CML 中的作用及其治疗意义。© 2024。作者获得 Springer Nature B.V. 的独家许可。

The current understanding emphasizes the intricate interplay between the Leukemic cell and its environment. Platelet-derived microparticles play a crucial role in facilitating intercellular communication and contribute to the complex landscape of cancer pathology. This study aimed to investigate the influence of platelet-derived microparticles on cell proliferation, apoptosis, and the expression of key genes, including P53, P21, Cyclin D1, Bax, and Bcl-2, within the context of a chronic myeloid leukemia cell line (K562).Platelet-derived microparticles were obtained through centrifugation at various speeds, and their concentration was quantified using the BCA assay. To determine the size and immunophenotypic characteristics of the PMPs, both the DLS technique and flow cytometry were employed. Cell proliferation was assessed using the MTT assay and hemocytometer, and cell cycle analysis was conducted through DNA content evaluation. Real-time PCR was utilized for gene expression analysis of Bax, Bcl-2, Cyclin D1, P53, and P21. Flow cytometry was employed to examine cell apoptosis. The findings revealed that platelet-derived microparticles have the ability to decrease proliferation of the K562 cell line, while not exerting an impact on apoptosis and cell cycle progression. Analysis through real-time PCR indicated an upregulation in the gene expression of P53, P21, and Bcl-2, accompanied by a downregulation in Bax and Cyclin D1.This investigation sheds light on the intricate relationship between chronic myeloid leukemia and its microenvironment, particularly the involvement of platelet-derived microparticles. The study underscores the potential of platelet-derived microparticles to influence cell behavior and gene expression, providing a deeper understanding of their role in CML and its therapeutic implications.© 2024. The Author(s), under exclusive licence to Springer Nature B.V.