结直肠癌（CRC）是指发生在结肠或直肠的高死亡率、高复发率的肿瘤。长链非编码RNA（lncRNA）的参与有助于CRC的治疗和预后评估，为患者的根治带来新的方向。目的 探讨lncRNA LINC01128（LINC01128）对结直肠癌细胞的病理机制和调控，并分析其潜在的预后价值。采用RT-qPCR评估122例结直肠癌患者组织和细胞标本中LINC01128的水平。通过 Kaplan-Meier 和 Cox 分析分析 LINC01128 在 CRC 中的临床意义和预后价值。使用 CCK8 和 Transwell 测定法研究 LINC01128 的体外功能。通过荧光素酶报告基因检测证实LINC01128与miR-363-3p之间的关系。LINC01128的过表达与CRC患者的TNM分期和淋巴结转移相关。沉默LINC01128可抑制CRC细胞的增殖和转移。此外，LINC01128直接靶向并负向调节miR-363-3p的表达，而miR-363-3p抑制剂恢复了LINC01128的抑制功能。作为CRC的独立预后因素，LINC01128的上调可预测不良预后并通过以下方式加速肿瘤恶化： miR-363-3p.© 2024。作者。

Colorectal cancer (CRC) refers to high-mortality tumors arising in the colon or rectum with a high rate of recurrence. The involvement of long non-coding RNAs (lncRNAs) contributes to the treatment and prognosis evaluation of CRC, and brings a new direction for the radical cure of patients. To identify the pathological mechanism and regulation of lncRNA LINC01128 (LINC01128) on CRC cells, and analyze its potential prognostic value.LINC01128 level in tissue and cell specimens from 122 CRC patients was evaluated by RT-qPCR. The clinical significance and prognostic value of LINC01128 in CRC were analyzed via Kaplan-Meier and Cox analysis. CCK8 and Transwell assays were used to study the function of LINC01128 in vitro. The relationship between LINC01128 and miR-363-3p was confirmed by luciferase reporter gene assay.The overexpression of LINC01128 is associated with TNM stage and lymph node metastasis in CRC patients. Silencing LINC01128 inhibited the proliferation and metastasis of CRC cells. In addition, LINC01128 directly targeted and negatively regulated the miR-363-3p expression, while miR-363-3p inhibitor restored the inhibitory function of LINC01128.As an independent prognostic factor of CRC, upregulation of LINC01128 predicts poor prognosis and accelerates tumor deterioration through miR-363-3p.© 2024. The Author(s).