众所周知，TH1 和 Treg 细胞分别发挥抗肿瘤活性和促肿瘤活性。因此，TH1 细胞抑制和 Treg 细胞过度激活有助于肿瘤的发展。光果甘草 (G. glabra) 具有多种免疫调节和抗肿瘤特性。利用乳腺癌 (BC) 模型探讨光果甘草提取物对 TH1 和 Treg 细胞相关不同参数的影响。四组 Balb/C 小鼠携带4T1细胞诱导的BC用盐水或G.glabra提取物以50、100和150mg/kg的剂量（分别为G.glabra-50、G.glabra-100和G.glabra-150）腹膜内处理。第26天处死动物后，检测脾脏TH1和Treg细胞的频率，血清IFN-γ、TGF-β和IL-12的水平，以及瘤内颗粒酶-B、T-bet和FOXP3的表达。与未治疗的肿瘤对照（UTC）组相比，G.glabra-50、G.glabra-100或G.glabra-150治疗增加了存活率、TH1细胞百分比和T-bet表达。相反，他们降低了 Treg 细胞的百分比和血清 TGF-β 水平。与UTC组相比，G.glabra-50和G.glabra-150治疗增加了血清IL-12水平。用 G. glabra-100 和 G. glabra-150 处理可增强颗粒酶 B 的表达。 G. glabra-150 处理提高了 IFN-γ 水平，而 G. glabra-50 处理降低了 FOXP3 表达。与用 G. glabra-100 治疗的小鼠相比，用 G. glabra-150 治疗的小鼠的 IL-12 水平较高。使用 G. glabra 提取物治疗患有 BC 的小鼠可提高存活率，减少肿瘤生长，并调节 T 细胞介导的免疫反应。

It is well-known that TH1 and Treg cells exert anti- and pro-tumorigenic activity, respectively. Thus, TH1 cell suppression together with Treg cell hyperactivation contribute to tumor development. Glycyrrhiza glabra (G. glabra) has various immunomodulatory and anti-tumorigenic properties.To explore the impacts of G. glabra extract on different parameters related to TH1 and Treg cells using a breast cancer (BC) model.Four groups of Balb/C mice bearing 4T1 cell-induced BC were treated intraperitoneally with either saline or G. glabra extract at dosages of 50, 100 and 150 mg/kg (G. glabra-50, G. glabra-100, and G. glabra-150, respectively). After sacrificing animals on day 26, the frequency of splenic TH1 and Treg cells, the levels of serum IFN-γ, TGF-β, and IL-12, and intra-tumoral expressions of granzyme-B, T-bet, and FOXP3 were assessed.Compared to untreated tumor control (UTC) group, treatment with G. glabra-50, G. glabra-100, or G. glabra-150 increased the survival rate, percentage of TH1 cells, and T-bet expression. Conversely, they reduced the percentage of Treg cells, and serum TGF-β levels. In comparison to the UTC group, treatment with G. glabra-50 and G. glabra-150 increased the serum IL-12 levels. Treatment with G. glabra-100 and G. glabra-150 boosted granzyme-B expression. Treatment with G. glabra-150 elevated IFN-γ levels, while treatment with G. glabra-50 decreased the FOXP3 expression. IL-12 levels were higher in mice treated with G. glabra-150 compared to those treated with G. glabra-100.Treatment of mice with BC using G. glabra extract improved survival rate, reduced tumor growth, and modulated T cell-mediated immune responses.