双酚 F (BPF) 是一种内分泌干扰化学物质，但有关其对甲状腺激素影响的信息尚未得到充分探索。另一方面，欧米伽 3 脂肪酸 (O3FA) 是抗氧化剂和抗细胞凋亡剂。因此，本研究探讨了O3FA在BPF诱导的甲状腺功能减退介导的雄性Wistar大鼠睾丸功能障碍中的作用和相关分子机制。 将二十（20）只雄性Wistar大鼠随机分为四组（n=5/组），即：控制组; BPF治疗组(30mg/kg的BPF)；和干预组（30mg/kg BPF 100mg/kg O3FA (BPF O3FA-L) 和 30mg/kg BPF 300mg/kg O3FA，持续 28 天）。低剂量和高剂量的 O3FA 改善了 BPF 诱导的甲状腺功能减退介导的精子减少质量、睾酮、黄体生成素、卵泡刺激素、过氧化氢酶、超氧化物歧化酶、总抗氧化能力和核因子红细胞2相关因子2以及雌激素、丙二醛、C反应蛋白、白细胞介素1β、半胱天冬酶3的增加。此外，O3FA 可预防 BPF 诱导的 Na /K -ATP 酶和 Ca2 -ATP 酶功能障碍、雌激素受体 β 过度表达以及肿瘤蛋白 P53 (p53)/b 细胞淋巴瘤 2 (Bcl-2) 失衡。这项研究表明，O3FA 改善了 BPF-通过预防质子泵功能障碍和 p53/BCl-2 失衡诱导甲状腺功能障碍介导的睾丸功能障碍。

Bisphenol F (BPF) is an endocrinedisrupting chemical, but information about its effect on thyroid hormones has not been fully explored. Omega 3 fatty acids (O3FA), on the other hand, are antioxidant and antiapoptotic agents. Therefore, this study explored the role and associated molecular mechanism of O3FA in BPF-induced hypothyroidism-mediated testicular dysfunction in male Wistar rats.Twenty (20) male Wistar rats were randomized into four groups (n=5/group), namely: the control group; the BPF treated group (30 mg/kg of BPF); and the intervention groups (30mg/kg BPF + 100mg/kg O3FA (BPF+O3FA-L) and 30mg/kg BPF + 300mg/kg of O3FA for 28 days).Low and high doses of O3FA ameliorated BPF-induced hypothyroidism-mediated reduction in sperm quality, testosterone, luteinizing hormone, follicle-stimulating hormone, catalase, superoxide dismutase, total antioxidant capacity, and nuclear factor erythroid 2-related factor 2 and increases in estrogen, malondialdehyde, c-reactive protein, interleukin 1 beta, caspase 3. Furthermore, O3FA prevented BPF-induced Na+/K+-ATPase and Ca2+-ATPase dysfunction, estrogen receptor beta overexpression, and tumor protein P53 (p53)/ b-cell lymphoma 2 (Bcl-2) imbalance.This study showed that O3FA ameliorated BPF-induced dysthyroidism-mediated testicular dysfunction by preventing proton pump dysfunction and p53/BCl-2 imbalance.