促进伤口愈合的传统方法可能并不总能产生令人满意的结果。相反，我们测试了新开发的光动力疗法 (PDT) 的有效性，该疗法使用载有聚乙二醇 (PEG) (MB-PEG) 水凝胶的亚甲蓝 (MB) 来加速小鼠伤口愈合过程。背部皮肤切口 6 毫米打孔器局部接受 MB-PEG 水凝胶和低强度红光激光照射，以评估受伤皮肤的再生过程。总共63只成年雄性CD1小鼠分为正常组（未处理）和其他伤口组，接受不同的激光治疗（650 ± 5 nm，功率强度为180 mW/cm2）、MB-PEG或PDT（MB-PEG其次是激光）。通过早期（48小时）和第21天晚期的皮肤组织学检查和促炎细胞因子测定来研究伤口愈合参数。48小时时，组织肉芽、炎症和血管生成过程的评分显着增加。与单独接受激光治疗的组相比，接受MB  PEG联合激光治疗的受伤组的情况有所改善。第 21 天，与其他组相比，MB  PEG 加激光治疗组的炎症明显改善。 48 h时，MB  PEG联合激光治疗组创面组血清中上调的肿瘤坏死因子（TNF）-α和白细胞介素（IL）-1β水平显着降低（P < 0.001）。与任何一种单一治疗相比，基于水凝胶的激光治疗可改善并加速伤口闭合。© 2024。作者。

Conventional approaches for enhancing wound healing may not always yield satisfactory results. Instead, we test the effectiveness of a newly developed photodynamic therapy (PDT) that uses methylene blue (MB) loaded with polyethylene glycol (PEG) (MB-PEG) hydrogel to accelerate wound healing process in mice.A dorsal skin incision with 6 mm punch which topically subjected to MB-PEG hydrogel and a low-level laser light of red light to assess the regeneration process of wounded skin. A total of 63 adult male CD1 mice divided into normal group (no treatment) and other wound groups received different treatments of laser (650 ± 5 nm and power intensity of 180 mW/cm2), MB-PEG, or PDT (MB-PEG followed by laser). The wound healing parameters were investigated by histological examination of the skin and measuring of proinflammatory cytokines at the early stage (48 h) and a late one on day 21.at 48 h, the score of tissue granulation, inflammation, and angiogenesis process were markedly improved in wounded groups that received MB + PEG combined with laser compared to the group treated with laser alone. On day 21, a significant improvement of the inflammation was detected in the group treated with MB + PEG plus laser compared to the other groups. At 48 h, the upregulated serum levels of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in the wound group were significantly (P < 0.001) reduced in the group treated with MB + PEG combined with laser.MB-PEG based hydrogel improves and accelerates wound closure in the context of laser compared to either single treatment.© 2024. The Author(s).