Pediocin PA-1 是一种从乳酸片球菌 PAC1.0 中提取的抗菌肽，由于其对食源性病原体单核细胞增生李斯特菌具有很强的抑制活性，因此具有作为食品防腐剂的潜在应用。本研究旨在从毕赤酵母中生产Pediocin PA-1，并评价其特性。将编码Pediocin PA-1的基因整合到毕赤酵母X33基因组中，建立菌株X33::ped，该菌株能够生产和分泌该肽。进入培养基。使用琼脂扩散试验检测 Pediocin PA-1 的抗菌活性。 Pediocin PA-1 的稳定性是根据其暴露于蛋白酶以及极端 pH 和温度后剩余的抗菌活性来确定的。使用单增李斯特菌感染的猪肉博洛尼亚证明了这​​种细菌素在食品保存中的潜在用途。还使用MTT法研究了Pediocin PA-1对一些癌细胞的抗癌活性。我们建立了能够产生Pediocin PA-1的酵母毕赤酵母X33::ped，该酵母对单核细胞增生李斯特菌和一些其他有害细菌具有抗菌活性。 Pediocin PA-1 在 100°C 下稳定，可耐受 pH 1-12 1 小时，但对胰蛋白酶、α-胰凝乳蛋白酶和蛋白酶 K 敏感。该肽可将猪肉博洛尼亚中单核细胞增生李斯特菌的数量减少 3.59 log 4°C 保存 7 天后的 CFU/g。最后，Pediocin PA-1 (25 µg/ml) 抑制 A549 和 Hela 癌细胞的增殖。我们成功地从毕赤酵母中生产出活性 Pediocin PA-1，并展示了其在食品保鲜和制药行业的潜在用途。© 2024。作者获得 Sociedade Brasileira de Microbiologia 的独家许可。

Pediocin PA-1, an antimicrobial peptide derived from Pediococcus acidilactici PAC1.0, has a potential application as a food preservative thanks to its strong inhibitory activity against the foodborne pathogen L. monocytogenes. This study aimed to produce Pediocin PA-1 from the yeast P. pastoris and evaluate its characteristics.Gene encoding Pediocin PA-1 was integrated into P. pastoris X33 genome to establish the strain X33::ped, which could produce and secrete this peptide into culture medium. The antimicrobial activity of Pediocin PA-1 was examined using agar diffusion assay. The stability of pediocin PA-1 was determined based on its remaining antibacterial activity after exposure to proteases and extreme pH and temperatures. The potential use of this bacteriocin in food preservation was demonstrated using the L. monocytogenes infected pork bologna. The anticancer activity of Pediocin PA-1 was also investigated on some cancer cells using MTT assay.We established the yeast P. pastoris X33::ped capable of producing pediocin PA-1 with antimicrobial activity against L. monocytogenes and some other harmful bacteria. Pediocin PA-1 was stable at 100˚C and resistant against pH 1-12 for 1 h, but susceptible to trypsin, α-chymotrypsin, and proteinase K. This peptide could reduce the number of L. monocytogenes in pork bologna by 3.59 log CFU/g after 7 days of storage at 4˚C. Finally, Pediocin PA-1 (25 µg/ml) inhibited the proliferation of A549 and Hela cancer cells.We succeeded in producing active Pediocin PA-1 from P. pastoris and demonstrated its potential use in food preservation and pharmaceutical industry.© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.