光动力疗法（PDT）继续遇到各种各样的障碍，这些障碍源于光敏剂保存和递送系统的无效、成像导航的缺乏以及抗氧化剂/缺氧的肿瘤微环境。在此，开发了一种用于可追踪 PDT 的多功能冷冻微针贴片（表示为 CMN-CCPH）。过氧化氢酶 (CAT) 诱导的氧气 (O2) 产生和 Cu2 介导的谷胱甘肽 (GSH) 消耗进一步放大了治疗效果。 CMN-CCPH由作为载体的低温微针（CMN）和负载血卟啉单甲醚（HMME）的CAT生物矿化磷酸铜纳米花（CCP NF）作为有效负载组成。重要的是，HMME和CAT的生物活性功能在CCPH和CMN的保护下可以得到最佳维持超过60天，从而增强生物利用度并显着增强PDT疗效。通过荧光 (FL)/光声 (PA) 双工实时成像监测的 HMME 和氧合血红蛋白饱和度 (sO2) 的体内可视化揭示了 CMN 传递的 CCPH 对于瘤内 HMME 和 O2 富集并降低全身毒性的显着影响。这种多功能 CMN 贴片在消除肿瘤方面表现出明显的功效，凸显了其广阔的临床前景。本文受版权保护。保留所有权利。本文受版权保护。版权所有。

Photodynamic therapy (PDT) continues to encounter multifarious hurdles, stemming from the ineffectual preservation and delivery system of photosensitizers, the dearth of imaging navigation, and the antioxidant/hypoxic tumor microenvironment. Herein, a versatile cryomicroneedle patch (denoted as CMN-CCPH) was developed for traceable PDT. The therapeutic efficacy was further amplified by catalase (CAT)-induced oxygen (O2) generation and Cu2+-mediated glutathione (GSH) depletion. The CMN-CCPH is composed of cryomicroneedle (CMN) as the vehicle and CAT-biomineralized copper phosphate nanoflowers (CCP NFs) loaded with hematoporphyrin monomethyl ether (HMME) as the payload. Importantly, the bioactive function of HMME and CAT could be optimally maintained under the protection of CCPH and CMN for a duration surpassing 60 days, leading to a bolstered bioavailability and notable enhancements in PDT efficacy. The in vivo visualization of HMME and oxyhemoglobin saturation (sO2) monitored by fluorescence (FL)/photoacoustic (PA) duplex real-time imaging unveiled the noteworthy implications of CMN-delivered CCPH for intratumoral enrichment of HMME and O2 with reduced systemic toxicity. This versatile CMN patch demonstrated distinct effectiveness in neoplasm elimination, underscoring its promising clinical prospects. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.