碳纳米管（CNT）有潜力作为药物和基因治疗的输送系统，特别是在癌症治疗中。姜黄素 (CUR) 和甲氨蝶呤 (MTX) 的联合给药在癌症治疗中显示出前景，因为它使用的药物更少，副作用也更少。本研究使用 MTX 结合白蛋白 (BSA) 纳米颗粒 (BSA-MTX) 来增强和评估 CUR 的效率。体外细胞毒性测试、DLS、TEM、FTIR、UV/Vis、SEM 和 DSC 研究评估了制剂的物理和化学性质。蛋白酶 K 用于强化 MTX 和 BSA 之间的酰胺键。研究结果证明了使用 f-MWCNT-CUR-BSA-MTX 作为载体在癌症治疗中有效共同递送 CUR 和 MTX 的功效。 MTT比色法用于评估化学和药物化合物的效果。使用 MTT 方法研究细胞分裂，以研究纯 MWCNT、纯 CUR、MTX-BSA 和 f-MWCNT-CUR-MTX-BSA 的效果。对细胞系的研究表明，姜黄素和MTX与CNT的组合可以增加和改善两种药物抗癌的功效。姜黄素和甲氨蝶呤药物组合同时对 MCF-7 细胞产生协同作用，这表明这些药物有可能用作预防和治疗乳腺癌的策略。此外，与纯姜黄素、纯 MTX-BSA、MTX 和单独的 f-MWCNT 相比，f-MWCNT-CUR-MTX-BSA 对癌症治疗具有显着效果，且毒性最小。碳纳米管具有高比表面积与体积比、高化学稳定性、化学吸附能力、对药物和生物分子的高容量以及同时多种药物负载等独特性能。 -BSA MTX 显着影响癌症治疗），是理想的靶向药物输送和高治疗效率的替代选择。© 2024。作者。

Carbon nanotubes (CNTs) have the potential to serve as delivery systems for medicinal substances and gene treatments, particularly in cancer treatment. Co-delivery of curcumin (CUR) and Methotrexate (MTX) has shown promise in cancer treatment, as it uses fewer drugs and has fewer side effects. This study used MTX-conjugated albumin (BSA)-based nanoparticles (BSA-MTX) to enhance and assess the efficiency of CUR. In-vitro cytotoxicity tests, DLS, TEM, FTIR, UV/Vis, SEM, and DSC studies assessed the formulations' physical and chemical properties. The Proteinase K enzyme was used to severe amidic linkages between MTX and BSA. The findings demonstrated the efficacy of using ƒ-MWCNT-CUR-BSA-MTX as a vehicle for efficient co-delivery of CUR and MTX in cancer treatment. The MTT colorimetric method was used to evaluate the effect of chemical and medicinal compounds. Cell division was studied using the MTT method to investigate the effect of pure MWCNT, pure CUR, MTX-BSA, and ƒ-MWCNT-CUR-MTX-BSA. Studies on cell lines have shown that the combination of curcumin and MTX with CNT can increase and improve the effectiveness of both drugs against cancer. A combination of drugs curcumin and methotrexate simultaneously had a synergistic effect on MCF-7 cells, which indicated that these drugs could potentially be used as a strategy for both prevention and treatment of breast cancer. Also, ƒ-MWCNT-CUR-MTX-BSA was found to have a significant effect on cancer treatment with minimal toxicity compared to pure curcumin, pure MTX-BSA, MTX, and ƒ-MWCNT alone. Unique properties such as a high ratio of specific surface area to volume, high chemical stability, chemical adsorption ability, high capacity of drug and biomolecules of carbon nanotubes, as well as multiple drug loading at the same time The combination of ƒ-MWCNT-CUR-BSA MTX significantly impacts cancer therapy), are desirable as an alternative option for targeted drug delivery and high therapeutic efficiency.© 2024. The Author(s).