本文采用铁离子掺杂的花石墨氮化碳（Fe-CN-3，能量供体）构建免疫传感器的基底，并采用氧化铜纳米立方体（Cu2O，能量受体）作为高效的ECL猝灭探针。基于新型共振能量转移供体-受体对，初步开发了一种用于可溶性细胞角蛋白19片段（Cyfra21-1）检测的夹心猝灭电化学发光（ECL）免疫传感器。 Fe-CN-3是一种兼具金属离子掺杂和形貌调控优点的氮化碳，用于ECL发光体，可提供更加优异的ECL性能，为氮化碳在电子学领域的应用和发展做出了重大贡献。 ECL生物传感器领域。猝灭剂Cu2O纳米立方体的规则形状、高比表面积和优异的生物相容性有利于二抗的标记和传感器的构建。同时，作为能量受体，Cu2O的紫外吸收光谱可以与能量供体的ECL发射光谱有效重叠，使其易于发生ECL-RET，从而获得优异的猝灭效果。供体-受体对的这些优点使传感器具有0.00005-100 ng/mL的宽检测范围和17.4 fg/mL（S/N = 3）的低检测限，这提供了新的方法和理论基础用于肺癌的临床检测。版权所有 © 2024 Elsevier B.V. 保留所有权利。

In this article, ferric ion-doped floral graphite carbon nitride (Fe-CN-3, energy donor) was used to construct the substrate of the immunosensor and copper oxide nanocubes (Cu2O, energy acceptor) were taken as an efficient ECL quenching probe. A sandwich quench electrochemiluminescence (ECL) immunosensor for soluble cytokeratin 19 fragment (Cyfra21-1) detection was preliminarily developed based on a novel resonant energy transfer donor-acceptor pair. Fe-CN-3, a carbon nitride that combines the advantages of metal ion doping as well as morphology modulation, is used in ECL luminophores to provide more excellent ECL performance, which makes a significant contribution to the application and development of carbon nitride in the field of ECL biosensors. The regular shape, high specific surface area and excellent biocompatibility of the quencher Cu2O nanocubes facilitate the labeling of secondary antibodies and the construction of sensors. Meanwhile, as an energy acceptor, the UV absorption spectrum of Cu2O can overlap efficiently with the energy donor's ECL emission spectrum, making it prone to the occurrence of ECL-RET and thus obtaining an excellent quenching effect. These merits of the donor-acceptor pair enable the sensor to have a wide detection range of 0.00005-100 ng/mL and a low detection limit of 17.4 fg/mL (S/N = 3), which provides a new approach and theoretical basis for the clinical detection of lung cancer.Copyright © 2024 Elsevier B.V. All rights reserved.