牛白血病病毒 (BLV) 包膜 (Env) 糖蛋白复合物在病毒感染性和塑造宿主免疫反应方面发挥着重要作用。本研究介绍了在稳定的 S2 昆虫细胞系中表达的可溶性弗林蛋白酶突变的 BLV Env 胞外域 (sBLV-EnvFm) 的生产和表征。我们纯化了一种 63 kDa 可溶性蛋白，对应于单体 sBLV-EnvFm，其主要呈现寡甘露糖和寡甘露糖 N-聚糖，具有高含量的核心岩藻糖结构。我们的结果表明，我们的重组蛋白可以从感染 BLV 的牛中的特异性抗体中识别出来，这表明其作为强大的诊断工具的潜力。此外，它在小鼠中引发的强大体液免疫反应表明它对开发基于亚单位的 BLV 疫苗的潜在贡献。© 2024。作者。

The Bovine Leukemia Virus (BLV) Envelope (Env) glycoprotein complex is instrumental in viral infectivity and shapes the host's immune response. This study presents the production and characterization of a soluble furin-mutated BLV Env ectodomain (sBLV-EnvFm) expressed in a stable S2 insect cell line. We purified a 63 kDa soluble protein, corresponding to the monomeric sBLV-EnvFm, which predominantly presented oligomannose and paucimannose N-glycans, with a high content of core fucose structures. Our results demonstrate that our recombinant protein can be recognized from specific antibodies in BLV infected cattle, suggesting its potential as a powerful diagnostic tool. Moreover, the robust humoral immune response it elicited in mice shows its potential contribution to the development of subunit-based vaccines against BLV.© 2024. The Author(s).