木鳖假种皮含有高含量的类胡萝卜素。该类胡萝卜素具有抗氧化、抗炎、抗肿瘤等多种药理特性。为了研究木鳖假种皮提取物对人结直肠癌细胞的抗癌活性及其相关机制。对结直肠癌细胞系HCT116和HT29进行处理分别使用 MTT/MTS 和集落形成测定分析木鳖假种皮提取物及其对细胞毒性和抗增殖的影响。然后，通过细胞死亡检测ELISA和流式细胞术进一步研究了抗增殖的相关机制。结果表明，处理后的细胞变得圆润，与邻近细胞失去接触，导致细胞活力降低。评估细胞毒性作用，对HCT116和HT29细胞的IC50分别为2.16 mg/mL和1.29 mg/mL，但在相同剂量下对正常HEK293没有毒性。此外，木鳖假种皮提取物显着抑制增殖能力，浓度增加效果更大。随后，验证了负责抑制增殖的细胞机制。它显示细胞凋亡诱导和细胞周期停滞。我们的研究结果表明，Gac 假种皮提取物可以诱导 HCT116 和 HT29 结直肠癌细胞的细胞凋亡和 S 期和 G2/M 期细胞周期停滞。

Gac aril contains high level of carotenoids. This carotenoid possesses several pharmacological properties including antioxidant, anti-inflammatory, and anti-tumor activities.To investigate the anti-cancer activity of Gac aril extract on human colorectal cancer cells and its related mechanisms.Colorectal cancer cell lines HCT116 and HT29 were treated with Gac aril extract and its effects on cytotoxicity and anti-proliferation were analyzed using the MTT/MTS and colony formation assay, respectively. Then, further related mechanisms responsible for anti-proliferation were investigated by cell death detection ELISA and Flow cytometry.The results showed that treated cells became rounded up and there was a loss of contact with neighboring cells, leading to a reduction of cell viability. The cytotoxic effects were evaluated IC50 for HCT116 and HT29 cells were 2.16 mg/mL and 1.29 mg/mL, respectively but it not toxic to normal HEK293 at the same dose. Moreover, Gac aril extract significantly inhibits proliferative ability with increasing concentrations having a greater effect. Subsequently, the cellular mechanism responsible for suppressive proliferation was validated. It shows apoptosis induction and arrest of cell cycle.Our findings demonstrated that Gac aril extract can induce apoptosis and arrest of cell cycle at S and G2/M phases in both HCT116 and HT29 colorectal cancer cells.