大多数肝细胞癌 (HCC) 患者的诊断发生在中晚期，有一些治疗方案可供选择。因此，迫切需要发现针对这种致命恶性肿瘤的额外辅助疗法。本研究旨在评估姜黄素 (C)、胡椒碱 (P) 和牛磺酸 (T) 组合作为佐剂对接受经动脉化疗的 HCC 患者血清 IFN-γ 水平、免疫表型和单核白细胞 (MNL) 分子特征的有效性化疗栓塞（TACE）。 收集 20 名接受 TACE 治疗的 HCC 患者治疗前（基线对照样本）和治疗后的血清和 MNL，每天服用 5 g 姜黄素胶囊、10 mg 胡椒碱和 0.5 mg 牛磺酸，连续三个月服用。 MNL 的免疫表型和分子特征分别通过流式细胞术和定量实时 PCR 确定。此外，通过ELISA对血清IFN-γ水平进行定量。接受CPT组合治疗后，与基础线对照样本相比，患者血清中IFN-γ水平显着升高。此外，与对照组相比，接受联合治疗的组在 MNL 中显示 PD-1 表达水平下调。 MNL 的免疫表型显示 CD4 CD25 细胞（调节性 T 淋巴细胞）显着下降。此外，临床病理学特征揭示了 CPT 组合对天冬氨酸转氨酶 (AST)、乳酸脱氢酶 (LDH) 和甲胎蛋白 (AFP) 水平的高度显着影响。本研究介绍了一种有前途的辅助 CPT 联合治疗作为天然药物，以加强对适合 TACE 治疗的 HCC 患者。

Diagnosis of the majority of hepatocellular carcinoma (HCC) patients occurs at intermediate to advanced stages, with a few curative therapeutic options being available. It is therefore strongly urgent to discover additional adjuvant therapy for this lethal malignancy. This study aimed to assess the effectiveness of curcumin (C), piperine (P) and taurine (T) combination as adjuvant agents on serum levels of IFN-γ, immunophenotypic and molecular characterization of mononuclear leukocytes (MNLs) in HCC patients treated with Transarterial chemoembolization (TACE).Serum and MNLs were collected from 20 TACE-treated HCC patients before (baseline-control samples) and after treatment with 5 g curcumin capsules , 10 mg piperine and 0.5 mg taurine taken daily for three consecutive months. Immunophenotypic and molecular characterization of MNLs were determined by flow cytometry and quantitative real time PCR, respectively. In addition, serum IFN-γ level was quantified by ELISA.After receiving treatment with CPT combination, there was a highly significant increase in IFN- γ levels in the sera of patients when compared to basal line control samples. Additionally, the group receiving combined therapy demonstrated a downregulation in the expression levels of PD-1, in MNLs as compared to controls. MNLs' immunophenotyping revealed a significant decline in CD4+CD25+cells (regulatory T lymphocytes). Furthermore, clinicopathological characteristics revealed a highly significant impact of CPT combination on aspartate aminotransferase (AST), lactate dehydrogenase (LDH) and alpha feto protein (AFP) levels.This study introduces a promising adjuvant CPT combined treatment as natural agents to enhance the management of HCC patients who are candidates to TACE treatment.