肝细胞癌（HCC）严重威胁人类健康。先前的研究表明，γ-谷氨酰转肽酶（GGT）与慢性损伤、肝纤维化和肝癌的发生密切相关，因此可能作为监测肝癌相关过程的潜在指标。在此，在结构优化的探针ETYZE-GGT的帮助下，在多个HCC相关模型中实现了远红荧光（FL）和光声（PA）模式的双峰成像。据我们所知，这项工作涵盖了最全面的模型，包括纤维化和发展的 HCC 过程以及前期诱导阶段（自身免疫性肝炎、药物性肝损伤、非酒精性脂肪肝）。 ETYZE-GGT 通过可视化 GGT 动态来报告 HCC 阶段，表现出稳定实用的监测性能。两种模式表现出工作一致性和互补性，具有高空间分辨率、精确的仪器和理想的生物相容性。配合现有的血清指标检测、病理染色等技术，ETYZE-GGT基本实现了对尽可能多的HCC分期进行临床前准确诊断的普遍应用。通过深入探讨GGT与HCC过程之间的机械相关性，特别是在前期诱导阶段，我们可以进一步提高HCC早期诊断和治疗的功效。版权所有©2024 Elsevier Ltd.保留所有权利。

Hepatocellular carcinoma (HCC) seriously threatens the human health. Previous investigations revealed that γ-glutamyltranspeptidase (GGT) was tightly associated with the chronic injury, hepatic fibrosis, and the development of HCC, therefore might act as a potential indicator for monitoring the HCC-related processes. Herein, with the contribution of a structurally optimized probe ETYZE-GGT, the bimodal imaging in both far red fluorescence (FL) and photoacoustic (PA) modes has been achieved in multiple HCC-related models. To our knowledge, this work covered the most comprehensive models including the fibrosis and developed HCC processes as well as the premonitory induction stages (autoimmune hepatitis, drug-induced liver injury, non-alcoholic fatty liver disease). ETYZE-GGT exhibited steady and practical monitoring performances on reporting the HCC stages via visualizing the GGT dynamics. The two modes exhibited working consistency and complementarity with high spatial resolution, precise apparatus and desirable biocompatibility. In cooperation with the existing techniques including testing serum indexes and conducting pathological staining, ETYZE-GGT basically realized the universal application for the accurate pre-clinical diagnosis of as many HCC stages as possible. By deeply exploring the mechanically correlation between GGT and the HCC process, especially during the premonitory induction stages, we may further raise the efficacy for the early diagnosis and treatment of HCC.Copyright © 2024 Elsevier Ltd. All rights reserved.