这项研究的重点是评估富含天然抗氧化剂的特级初榨橄榄油和橄榄果配方作为潜在的营养补充剂，用于缓解分子病理生理学受氧化应激和炎症影响的疾病（例如阿尔茨海默病）的症状和长期后果除了评估暴露于培养物制剂的人肝细胞癌 HepG2 细胞的细胞活力和增殖能力外，对从细胞培养物中分离的 RNA 样品使用定量实时 PCR 并应用方法，还认为肝毒性是一种额外的安全措施进行靶向分子分析，通过基因表达谱揭示潜在的通路效应。此外，本工作研究的配方将天然提取物的添加与化学形式进行了对比，并评估了抗氧化剂递送模式对细胞毒性的影响。结果表明，细胞毒性极小，对 HepG2 细胞培养物中的代谢分子途径具有显着的有益影响，从而为在膳食补充剂中使用橄榄油和抗氧化剂来创新治疗策略的方法，可以最大限度地减少氧化应激和炎症信号对患有 AD 等疾病的个体的长期影响。总体而言，实验设计和获得的数据支持应用的概念创新的分子方法和研究技术，可显着推进营养补充剂和膳食产品的交付以及科学影响和验证，以改善公共健康和医疗保健结果。版权所有 © 2024 Prodromou、Chatzopoulou、Saiti、Giannopoulos-Dimitriou、Koudoura、Pantazaki 、Chatzidimitriou、Vasiliou 和 Vizirianakis。

This study focuses on the assessment of extra virgin olive-oil and olive fruit-based formulations enriched with natural antioxidants as potential nutritional supplements for alleviating symptoms and long-term consequences of illnesses whose molecular pathophysiology is affected by oxidative stress and inflammation, such as Alzheimer's disease (AD).Besides evaluating cell viability and proliferation capacity of human hepatocellular carcinoma HepG2 cells exposed to formulations in culture, hepatotoxicity was also considered as an additional safety measure using quantitative real-time PCR on RNA samples isolated from the cell cultures and applying approaches of targeted molecular analysis to uncover potential pathway effects through gene expression profiling. Furthermore, the formulations investigated in this work contrast the addition of natural extract with chemical forms and evaluate the antioxidant delivery mode on cell toxicity.The results indicate minimal cellular toxicity and a significant beneficial impact on metabolic molecular pathways in HepG2 cell cultures, thus paving the way for innovative therapeutic strategies using olive-oil and antioxidants in dietary supplements to minimize the long-term effects of oxidative stress and inflammatory signals in individuals being suffered by disorders like AD.Overall, the experimental design and the data obtained support the notion of applying innovative molecular methodologies and research techniques to evidently advance the delivery, as well as the scientific impact and validation of nutritional supplements and dietary products to improve public health and healthcare outcomes.Copyright © 2024 Prodromou, Chatzopoulou, Saiti, Giannopoulos-Dimitriou, Koudoura, Pantazaki, Chatzidimitriou, Vasiliou and Vizirianakis.