山楂籽是水果加工的副产品，由于这种原材料的加工规模，它们可以成为生物活性化合物的重要来源。这项工作是首次报道山楂籽的酚类成分及其抗氧化、抗炎、抗糖尿病、抗肥胖和抗癌活性。采用UPLC-ESI-TQD-MS/MS方法从6种种子的分离酚组分中鉴定出23种酚类化合物，其中关键化合物包括B型原花青素二聚体。受试物种的种子表现出较高的抗氧化活性（主要通过清除O2•-和OH•自由基）、抗炎（主要通过LOX抑制）、抗糖尿病、抗肥胖和抗癌活性，其中抗氧化活性最高。结肠癌细胞（Dld-1 系），对健康结肠上皮细胞（CCD841CoN）没有活性。该活性显着​​依赖于所分析的山楂品种，并且根据 PCA 分析，依赖于 flavan-3-ols 的含量。这些发现为山楂种子的工业利用提供了理论基础。© 2024。作者。

Hawthorn seeds are a by-product of fruit processing and due to the scale of processing of this raw material, they can be an important source of bioactive compounds. This work is the first report on the phenolic composition of hawthorn seeds and their antioxidant, anti-inflammatory, antidiabetic, antiobesity and anticancer activities. In the isolated phenolic fraction of six seed species, 23 phenolic compounds were identified using the UPLC-ESI-TQD-MS/MS method, the key ones of which included the B-type procyanidin dimer. The seeds of the tested species showed high antioxidant activity (mainly by scavenging O2•- and OH• radicals), anti-inflammatory (mainly through LOX inhibition), anti-diabetic, anti-obesity and anti-cancer, with the highest activity against colon cancer cells (Dld-1 line), showing no activity against healthy colon epithelial cells (CCD841CoN). This activity was significantly dependent on the analyzed hawthorn species and, according to PCA analysis, on the content of flavan-3-ols. These discoveries provided the theoretical basis for the possibility of industrial use of hawthorn seeds.© 2024. The Author(s).