为了探讨 miRNA-28-3p 如何靶向 BIN1 并影响鼻咽癌细胞的生物学行为，并评估其作为鼻咽癌预测生物标志物的潜力。我们研究了 2021 年 1 月至 2022 年 1 月期间接受手术的 100 名鼻咽癌患者。分析肿瘤和邻近的正常区域。参与者被分为两组：一组患有鼻咽癌，另一组患有邻近正常组织。此外，鼻咽癌细胞系CNE-2被分为三组：未处理的对照、NC质粒的阴性对照和用miRNA-28-3p抑制剂处理的测试组。关键技术包括PCR、Western blotting、CCK-8、流式细胞术，重点研究miRNA-28-3p与BIN1之间的相互作用及其对鼻咽癌的预测意义。鼻咽癌组织中miRNA-28-3p的水平显着高于癌旁组织正常组织 (P < .05)。 miRNA-28-3p 对鼻咽癌的预测性能 AUC > 0.75，敏感性和特异性均超过 70% (P < .001)。在鼻咽癌细胞中，与正常细胞相比，miRNA-28-3p 水平显着升高 (P < .05)。转染 miRNA-28-3p 抑制剂可增加细胞凋亡和 BIN1 蛋白水平，同时显着降低细胞增殖、侵袭和迁移 (P < .05)。miRNA-28-3p 在鼻咽癌中过表达，抑制肿瘤细胞增殖、迁移、和侵袭，同时通过靶向 BIN1 促进细胞凋亡。 miRNA-28-3p 表达水平可作为预测鼻咽癌的敏感指标，证实了其作为诊断生物标志物的潜力，并强调了这些发现在增强对鼻咽癌的理解和临床管理方面的重要性。

To explore how miRNA-28-3p targets BIN1 and affects the biological behavior of nasopharyngeal carcinoma cells, and to evaluate its potential as a predictive biomarker for NPC.We studied 100 nasopharyngeal carcinoma patients who underwent surgery between January 2021 and January 2022. Tissues from tumors and adjacent normal areas were analyzed. Participants were categorized into two groups: one with nasopharyngeal carcinoma and another with adjacent normal tissue. Additionally, the nasopharyngeal carcinoma cell line CNE-2 was divided into three groups: an untreated control, a negative control with NC plasmid, and a test group treated with a miRNA-28-3p inhibitor. Key techniques included PCR, Western blotting, CCK-8, flow cytometry, focusing on the interactions between miRNA-28-3p and BIN1 and their predictive significance for NPC.miRNA-28-3p levels were significantly higher in nasopharyngeal carcinoma tissues compared to adjacent normal tissues (P < .05). The predictive performance of miRNA-28-3p for NPC featured an AUC > 0.75 with sensitivity and specificity both exceeding 70% (P < .001). In nasopharyngeal carcinoma cells, miRNA-28-3p levels were significantly elevated compared to normal cells (P < .05). Transfection with the miRNA-28-3p inhibitor increased apoptosis and BIN1 protein levels while reducing cell proliferation, invasion, and migration significantly (P < .05).miRNA-28-3p is overexpressed in nasopharyngeal carcinoma and inhibits tumor cell proliferation, migration, and invasion, while promoting apoptosis by targeting BIN1. The level of miRNA-28-3p expression serves as a sensitive indicator for predicting nasopharyngeal carcinoma, affirming its potential as a diagnostic biomarker and underscoring the significance of these findings in enhancing understanding and clinical management of NPC.