酪氨酸蛋白激酶SYK由SYK基因编码，是一种非受体型蛋白激酶，通过免疫受体介导免疫信号转导。酪氨酸蛋白激酶 SYK 表达与各种炎症性疾病、癌症和神经退行性疾病的发生有关。酪氨酸蛋白激酶 SYK 研究的可重复性将有助于阐明其引起神经炎症的机制及其作为治疗阿尔茨海默病的新靶点的潜力。高质量酪氨酸蛋白激酶 SYK 的出现将促进这一点。在这项研究中，我们使用基于比较敲除细胞系和同基因亲本对照读数的标准化实验方案，对 13 种用于蛋白质印迹、免疫沉淀和免疫荧光的酪氨酸蛋白激酶 SYK 商业抗体进行了表征。我们鉴定了许多高性能抗体，并鼓励读者使用本报告作为指南，选择最适合其特定需求的抗体。版权所有：© 2023 Alshafie W 等人。

Tyrosine-protein kinase SYK, encoded by the SYK gene, is a non-receptor type protein kinase which mediates immune signal transduction through immunoreceptors. Tyrosine-protein kinase SYK expression has been associated with the development of various inflammatory diseases, cancer and neurodegenerative conditions. The reproducibility of tyrosine-protein kinase SYK research would help elucidate the mechanism in which it causes neuroinflammation as well as its potential as a novel target to treat Alzheimer's disease. This would be facilitated with the availability of high-quality tyrosine-protein kinase SYK. In this study, we characterized thirteen tyrosine-protein kinase SYK commercial antibodies for Western Blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.Copyright: © 2023 Alshafie W et al.