本研究的目的是探讨重楼素Ⅶ (PP Ⅶ) 对弥漫性大 B 细胞淋巴瘤 (PLBCL) 细胞系 U2932 和 SUDHL-4 增殖、凋亡和细胞周期的影响。将DLBCL细胞系分为对照组和PPⅦ组，采用MTT法、流式细胞术、Western blotting等方法进行实验。结果显示，与对照组相比，PPⅦ显着抑制U2932和SUDHL-4的增殖。细胞（P<0.05）。凋亡检测结果显示，0.50和1.00 µmol/L PPⅦ处理后，两种细胞的凋亡率均显着升高（P<0.05），凋亡相关蛋白上调，Bcl-2蛋白水平下调（P<0.05）。细胞周期分析显示，PPⅦ处理导致G0/G1期细胞增多（P<0.05），G2/M期细胞减少（P<0.05），cyclin D1、CDK4、CDK6和survivin显着下调蛋白表达量（P<0.05）。总之，PPⅦ通过抑制DLBCL细胞增殖、促进细胞凋亡、诱导G0/G1期阻滞发挥抗淋巴瘤作用。

The aim of this study was to investigate the effects of polyphyllin Ⅶ (PP Ⅶ) on proliferation, apoptosis, and cell cycle of diffuse large B-cell lymphoma (PLBCL) cell lines U2932 and SUDHL-4. The DLBCL cell lines were divided into a control group and a PPⅦ group, and experiments were conducted using MTT assay, flow cytometry, and Western blotting.Results showed that compared with the control group, PPⅦ significantly inhibited the proliferation of U2932 and SUDHL-4 cells (P<0.05). Apoptosis assays demonstrated that treatment with 0.50 and 1.00 µmol/L PP Ⅶ significantly increased the apoptosis rates of both cell lines (P<0.05), upregulated apoptosis-related proteins, and downregulated Bcl-2 protein level (P<0.05). Cell cycle analysis revealed that PPⅦ treatment led to an increase in G0/G1-phase cells (P<0.05) and a decrease in G2/M-phase cells (P<0.05), significantly downregulated cyclin D1, CDK4, CDK6, and survivin protein expression (P<0.05). In conclusion, PPⅦ exerted anti-lymphoma effects by inhibiting proliferation, promoting apoptosis, and inducing G0/G1 phase arrest in DLBCL cells.