钠电压门控通道β亚基4 (SCN4B) 在多种肿瘤中发挥抑制作用。然而，SCN4B在非小细胞肺癌（NSCLC）中的作用尚不清楚。本研究旨在探讨SCN4B在NSCLC患者中的表达情况及其与预后的相关性。首先，利用癌症基因组图谱（TCGA）数据库对非小细胞肺癌（NSCLC）中SCN4B的表达进行分析。然后，使用R软件鉴定差异表达基因（DEG）。接下来，使用 R 包 clusterProfiler 分析 DEG 富集途径。通过 STRING 分析揭示了蛋白质-蛋白质相互作用网络。热图显示 SCN4B 的表达存在显着差异。进一步的分析包括检查 SCN4B 在泛癌环境中的表达及其与 NSCLC 中 24 种免疫细胞的相关性。随后利用实时定量聚合酶链反应（qRT-PCR）、Western Blot、免疫组化和临床数据来验证SCN4B在NSCLC患者中的表达和预后价值。非小细胞肺癌组织中SCN4B mRNA的表达显着降低高于邻近正常组织 (p < 0.001)。临床相关分析证实其与临床病理的相关性。基因集富集分析（GSEA）和肿瘤免疫相关分析表明，SCN4B参与NSCLC相关的京都基因和基因组百科全书（KEGG）通路并参与免疫浸润。 qRT-PCR、Western Blot和免疫组化也证实SCN4B在NSCLC患者中表达下调，并与不良预后相关。SCN4B在NSCLC患者的肿瘤组织中表达下调，并与不良预后相关。Copyright© Bentham Science Publishers；如有任何疑问，请发送电子邮件至 epub@benthamscience.net。

Sodium voltage-gated channel beta subunit 4 (SCN4B) plays a suppressive role in various tumors. However, the role of SCN4B in non-small cell lung cancer (NSCLC) is not yet clear. This study aims to investigate the expression of SCN4B in NSCLC patients and its correlation with prognosis.Firstly, the expression of SCN4B in non-small cell lung cancer (NSCLC) was analyzed using The Cancer Genome Atlas (TCGA) database. Then, differential expression genes (DEGs) were identified using R software. Next, DEG enrichment pathways were analyzed using the R package clusterProfiler. Protein-protein interaction networks were revealed through STRING analysis. A heatmap showed significant differential expression of SCN4B. Further analysis included examining SCN4B expression in a pan-cancer context and its correlation with 24 types of immune cells in NSCLC. Subsequently, quantitative real-time polymerase chain reaction (qRT-PCR), Western Blot, immunohistochemistry, and clinical data were used to validate SCN4B expression and prognostic value in NSCLC patients.SCN4B mRNA expression in non-small cell lung cancer tissues was significantly lower than in adjacent normal tissues (p < 0.001). Clinical correlation analysis confirmed its association with clinical pathology. Gene set enrichment analysis (GSEA) and tumor immune-related analyses indicated that SCN4B is involved in NSCLC-related Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and participates in immune infiltration. qRT-PCR, Western Blot, and immunohistochemistry also confirmed that SCN4B is downregulated in NSCLC patients and is associated with poor prognosis.SCN4B is downregulated in tumor tissues of NSCLC patients and is associated with a poor prognosis.Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.