芥子油苷是最具争议但又被忽视的一类植物化学物质。这些是生物活性较低的中间植物化学物质。但一旦植物受到任何伤害——人为的、昆虫造成的或自然的——奇迹就会发生。该化合物被分解成更小的植物化学物质，称为芥子油苷水解产物（腈、异硫氰酸盐和硫氰酸盐）。这些水解产品就像时尚界的终结者。这些化合物具有自然界中最高的生物活性之一。全球研究人员将这些化合物与各种生物活性（抗癌、抗氧化、杀虫、除草等）联系起来。本文的目的是提供批判性评论，以强调这些被忽视的化合物的一些重要生物活性并促进研究人员至少给这些化合物一个在黑暗中发光的机会。这篇评论是根据对过去五年（2018-2023）的可获取文献的分析而写的，除了一些至关重要的例外。该评论强调了简短的内容芥子油苷及其水解的背景。我们努力涵盖该化合物的大部分生物学特性。特别强调了该化合物的抗癌活性及其相关机制的详细信息。考虑到芥子油苷水解产物的广泛生物活性，有必要将其视为一种有前景的药物化合物。更多的芥子油苷水解产物——与萝卜硫素类似的方式——可以进行阶段试验。详细讨论了芥子油苷水解产物的生产机理途径和相关的生物活性。使用所涉及的机制进一步解释了生物活性。© 作者 2024。由牛津大学出版社代表 AOAC INTERNATIONAL 出版。版权所有。如需权限，请发送电子邮件至：journals.permissions@oup.com。

Glucosinolates are the most controversial yet ignored class of phytochemicals. These are the middleman phytochemicals which have low bioactivity. But once there is any injury in the plant-manmade, insect caused, or natural-a magic happens. The compound is broken down into smaller phytochemicals referred to as glucosinolate hydrolytic products (nitriles, isothiocyanates, and thiocyanates). These hydrolytic products are like show stopper of fashion industry. These compounds have one of the highest bioactivity in the nature. These have been associated with a varied range of bioactivities (anticancer, antioxidant, insecticidal, weedicide, etc) by researchers across the globe.The objective of the current article is to provide a critical review to highlight some of the important bioactivities of these ignored compounds and for promoting researchers to atleast give these compounds a chance-to glow in the dark.This review has been written from analysis of accessible literature mostly from the last 5 years (2018-2023), with some critically essential exceptions.The review highlighted brief background of glucosinolates and its hydrolysis. Effort were made to include most of the biological properties of the compound. Special emphasis has been given to the anticancer activities of the compound with details of involved mechanism.Considering the wide array of bioactivities of glucosinolate hydrolytic products, it is essential to consider it as a prospective medicinal compound. More glucosinolate hydrolytic products-in similar manner as sulforaphane-can be proceeded to phase trials.The mechanistic pathway for production of glucosinolate hydrolytic products and related biological activities have been discussed in details. The bioactivities have been further explained using the involved mechanism.© The Author(s) 2024. Published by Oxford University Press on behalf of AOAC INTERNATIONAL. All rights reserved. For permissions, please email: journals.permissions@oup.com.