弓蛔虫病是一种人畜共患寄生虫感染，在世界范围内分布，对人类健康影响很大。现有药物的临床解决方案有限，因此治疗具有挑战性。槲皮素具有生物和药理特性，包括抗寄生虫、抗氧化和抗癌活性，是当前药物的可能替代品。海洋无脊椎动物可以产生大量不同的分子，其中许多是具有独特特征的生物活性物质。在这项研究中，我们评估了槲皮素和仙女座（水母）毒液对犬弓蛔虫第三幼虫的体外杀线虫作用。在装有 Roswell Park Memorial Institute-1640 培养基的微孔板中，将幼虫与槲皮素乙醇提取物（0.01、0.02、0.05、0.08、0.1、0.25 和 0.5mM/mL）和仙女座毒液水提取物（15、20 、25、30、35、40 和 60μg/mL）来评估其杀幼虫效果。与阿苯达唑中培养的幼虫相比，扫描电子显微镜研究了两种提取物的致死浓度 (LC90) 对培养幼虫体壁的可能影响。我们的研究揭示了与对照组和阿苯达唑治疗组相比，槲皮素和仙女座蛇毒暴露对犬毛蝽第三幼虫死亡率和超微结构的影响。© 2024 Wiley periodicals LLC。

Toxocariasis is a zoonotic parasitic infection with worldwide distribution and high impact on human health. It has a limited clinical resolution with the available drugs, making it challenging to treat. Quercetin, which possesses biological and pharmacological qualities including antiparasitic, antioxidant, and anticancer activities, is a possible substitute for the current medications. Marine invertebrates can produce a vast array of different molecules, many of which are biologically active substances with distinct characteristics. In this study, we assessed the in vitro nematocidal effect of both quercetin and venom of Cassiopea andromeda (jellyfish) against third larvae of Toxocara canis. In microplates with Roswell Park Memorial Institute-1640 medium, larvae were incubated with ethanolic extract of quercetin (0.01, 0.02, 0.05, 0.08, 0.1, 0.25, and 0.5 mM/mL) and water extract of C. andromeda venom (15, 20, 25, 30, 35, 40, and 60 µg/mL) to evaluate their larvicidal effect. A scanning electron microscopy has investigated the possible effect of lethal concentration (LC90) of both extracts on the body wall of cultivated larvae, in comparison with those cultivated in albendazole. Our study revealed the effects of both quercetin and C. andromeda venom exposure on the mortality rate and the ultrastructure of T. canis third larva in comparison with control and albendazole-treated groups.© 2024 Wiley Periodicals LLC.