牙周病与免疫失调有关，释放的细胞因子会加剧 20​​19 年冠状病毒病 (COVID-19) 相关的细胞因子风暴，进一步恶化相关不良后果。目前尚缺乏调查患有牙周病的 COVID-19 患者细胞因子水平的具体研究。检查这些情况之间的相关性有助于对风险类别进行分类、确定转诊和加强口腔卫生方案。目前的研究旨在评估患有和不患有牙周病的COVID-19阳性患者唾液中的细胞因子水平。对26名COVID-19阳性患者进行了牙周检查、唾液采集，并通过细胞因子珠评估细胞因子水平基于多重测定，使用荧光编码珠和流式细胞术（BD FACS LSRFortessa）。评估了 11 种细胞因子（白介素 [IL]2、4、6、10、17A 和干扰素-γ (IFN-γ)、肿瘤坏死因子-α (TNF-α)、趋化因子配体 2（CCL2/单核细胞趋化蛋白- 1)、C-X-C 基序趋化因子配体 (CXCL) 8/IL 8、CXCL 9/单核因子诱导的 γ 干扰素 [MIG]）和 CXCL 10（趋化因子 IFN-γ 诱导蛋白 10 kDa）。还将招募的受试者的细胞因子水平与文献中报告的仅针对健康、COVID-19 和牙周病的唾液细胞因子水平进行了图形比较。 在 26 名 COVID-19 阳性患者中，有 17 人患有牙周病。与文献中报告的健康、单独牙周病或单独 COVID-19 的值相比，患有这两种疾病的患者的所有细胞因子水平均升高。然而，招募的受试者之间的 IL-2、IL-4、IL-6、IL-10、IL-17A、IFN-gamma、TNF-α、CCL2、CXCL 8 和 CXCL 10 没有统计学差异。研究发现，牙周健康、COVID-19 阳性受试者的细胞因子水平较高 (P = 0.01)。牙周疾病可能会导致 COVID-19 诱发的细胞因子风暴，并可能放大其影响。版权所有：© 2024 印度牙周病学会。

Periodontal disease is associated with immune dysregulation, and cytokines released can add on to the coronavirus disease 2019 (COVID-19)-associated cytokine storm, further worsening the related adverse outcomes. Specific studies investigating cytokine levels in COVID-19 patients with periodontal disease are lacking. Examining the correlation between these conditions could aid in categorizing risk categories, determining referrals, and strengthening oral hygiene protocols. The current study sought to evaluate cytokine levels in the saliva of COVID-19-positive patients with and without periodontal disease.Twenty-six COVID-19-positive patients were subjected to periodontal examination, saliva collection, and assessment of cytokine levels through cytokine bead-based multiplex assay, using fluorescence-encoded beads with flow cytometry (BD FACS LSRFortessa). Eleven cytokines were assessed (interleukin [IL] 2, 4, 6, 10, 17A, and interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-α), chemokine ligand 2 (CCL2/monocyte chemoattractant protein-1), C-X-C motif chemokine ligand (CXCL) 8/IL 8, CXCL 9/monokine-induced gamma interferon [MIG]), and CXCL 10 (chemokine IFN-gamma inducible protein 10 kDa). The cytokine levels of the recruited subjects were also compared graphically with the salivary cytokine levels reported in the literature for health, COVID-19, and periodontal disease alone.Out of 26 COVID-19-positive patients, 17 had periodontal disease. Levels of all cytokines were raised in patients with both diseases when compared to values reported in literature for health, periodontal disease alone, or COVID-19 alone. However, there was no statistical difference among the recruited subjects for IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-gamma, TNF-α, CCL2, CXCL 8, and CXCL 10. MIG levels were found to be higher in periodontally healthy, COVID-19-positive subjects (P = 0.01).Periodontal disease might contribute to the COVID-19-induced cytokine storm, potentially amplifying its impact.Copyright: © 2024 Indian Society of Periodontology.