睾丸扭转是泌尿外科急症，如果不及时诊断，可能会导致缺血性损伤和睾丸丧失。据报道，原花青素具有抗炎和抗氧化特性。本研究旨在探讨原花青素 (P) 在扭转/扭转 (T/D) 诱导的大鼠睾丸缺血/再灌注 (I/R) 损伤中对睾丸的可能影响。将 40 只大鼠分为 4 组（每组 10 只）：假手术组（sham）、I/R、I/R P100（100mg/kg，扭转前 30 分钟）和 I/R P200（200mg）。 /kg，扭转前30分钟）。通过顺时针 720° 扭转 3 小时，然后扭转 3 小时，对左侧睾丸进行睾丸 T/D。在 I/R 组中，与对照组相比，丙二醛 (MDA) 水平升高，谷胱甘肽 (GSH)、维生素 C (Vit C)、谷胱甘肽过氧化物酶 (GPx)、葡萄糖-6-磷酸脱氢酶 (G6PD) 值降低与假手术组相比（p<0.001）。此外，在细胞中检测到裂解的 caspase-3 和 Bcl2 相关 X 蛋白 (Bax) 的表达增加，B 细胞淋巴瘤 2 (Bcl-2) 和增殖细胞核抗原 (PCNA) 的表达减少。 I/R 组 (p<0.001)。组织病理学结果显示，I/R 组睾丸的 Johnsen 和 Cosentino 评分不规则（p<0.001）。原花青素治疗导致 MDA、cleaved caspase-3 和 Bax 水平降低，GSH、Vit C、GPx、G6PD、Bcl-2 和 PCNA 值增加。此外，约翰森和科森蒂诺重新排列了分数。目前的研究结果揭示了原花青素以其抗氧化和抗凋亡特性对睾丸扭转/扭转引起的缺血/再灌注引起的器官损伤具有保护和治疗作用。版权所有 © 2024 Elsevier Ltd. 保留所有权利。

Testicular torsion is an urological emergency and can lead to ischemia damage and testicular loss if not diagnosed in time. Proanthocyanidin is reported to have anti-inflammatory and antioxidant properties. The current study aimed to examine the possible effects of proanthocyanidin (P) on the testis in torsion/detorsion (T/D)-induced testicular ischemia/reperfusion (I/R) injury in rats. Forty rats were divided into four groups (n=10 for each): sham-operated (sham), I/R, I/R + P100 (100 mg/kg, 30 min before torsion), and I/R + P200 (200 mg/kg, 30 min before torsion). Testicular T/D was performed on the left testicle by 3 hours of torsion at 720° clockwise, followed by 3 hours of detorsion. In the I/R group, an increase in malondialdehyde (MDA) levels and a decrease in glutathione (GSH), vitamin C (Vit C), glutathione peroxidase (GPx), glucose-6-phosphate dehydrogenase (G6PD) values were determined compared to the sham group (p<0.001). Moreover, an increase in the expression of cleaved caspase-3 and Bcl2-associated X protein (Bax), a decrease in the expression of B-cell lymphoma 2 (Bcl-2) and proliferating cell nuclear antigen (PCNA) were detected in the I/R group (p<0.001). Histopathologically, it was determined that the Johnsen and Cosentino scores of the testicles were irregular in the I/R group (p<0.001). Proanthocyanidin treatment caused a decrease in MDA, cleaved caspase-3 and Bax levels and an increase in GSH, Vit C, GPx, G6PD, Bcl-2 and PCNA values. Additionally, Johnsen and Cosentino rearranged the scores. The present findings revealed the protective and curative effects of proanthocyanidin in organ damage due to testicular torsion/detorsion-induced ischemia/reperfusion with their antioxidative and antiapoptotic properties.Copyright © 2024 Elsevier Ltd. All rights reserved.