MicroRNA (miRNA) 是一类小 RNA 基因，在癌症生物学调控中发挥重要作用。关于 microRNA-505-3p (miR-505-3p) 在癌症发生和进展中的作用已有大量研究，但 miR-505-3p 在上皮性卵巢癌 (EOC) 中的功能尚未完全阐明。通过miRNA表达数据集的比较分析来选择差异表达的miRNA。应用实时定量聚合酶链反应检测RNA的表达水平，同时进行蛋白质印迹和免疫荧光染色检测目的蛋白的表达水平。通过伤口愈合和跨孔实验评估 EOC 细胞的运动能力。通过双荧光素酶实验研究miRNA与其直接靶基因的结合和调控关系。我们的结果表明，miR-505-3p 在复发性 EOC 中上调，通过调节细胞上皮间质转化显着抑制 EOC 细胞运动。此外，我们的结果表明，EOC 细胞中 miR-505-3p 直接结合到其 3'-URT 中，PEAK1 表达受到抑制。重要的是，PEAK1 的敲低减弱了 mi-505-3p 抑制剂对 EOC 细胞迁移和侵袭的影响。总之，我们的研究结果表明 miRNA-505-3p 通过靶向 PEAK1 抑制 EOC 细胞运动。© 2024 作者。 《生物化学和分子毒理学杂志》由 Wiley periodicals LLC 出版。

MicroRNAs (miRNAs) are a class of small RNA genes with important roles in cancer biology regulation. There are considerable studies regarding the roles of microRNA-505-3p (miR-505-3p) in cancer development and progression, but the function of miR-505-3p in epithelial ovarian cancer (EOC) has not been fully clarified. Comparative analysis of miRNA expression data set was used to select differentially expressed miRNAs. Quantitative real-time polymerase chain reaction was applied to detect expression levels of RNAs, while western blot and immunofluorescence staining were performed to detect expression levels of proteins of interest. The motility of EOC cells was assessed by wound healing and transwell assays. The binding and regulating relationship between miRNA and its direct target gene was investigated by dual-luciferase assay. Our results show that miR-505-3p was upregulated in recurrent EOC, which significantly inhibits EOC cell motility via modulating cell epithelial-mesenchymal transition. Furthermore, our results indicated that PEAK1 expression was inhibited by direct binding of miR-505-3p into its 3'-URT in EOC cells. Importantly, knockdown of PEAK1 attenuated the effect of mi-505-3p inhibitor on EOC cell migration and invasion. In conclusion, our findings indicate that miRNA-505-3p inhibits EOC cell motility by targeting PEAK1.© 2024 The Author(s). Journal of Biochemical and Molecular Toxicology published by Wiley Periodicals LLC.