乳腺癌干细胞（BCSC）作为一种肿瘤细胞能够自我再生，导致细胞凋亡抵抗和癌症复发。据报道，BCSC 含有较低水平的与干性能力相关的活性氧 (ROS)。苏木因其对多种癌细胞的化学预防功效而被提出。本研究旨在评估苏木提取物（CSE）对 MDA-MB-231 及其 BCSC 的细胞毒性、细胞凋亡诱导、ROS 生成和干性标记物的影响。苏木在甲醇浸渍下提取。磁激活细胞分选用于根据 CD44 和 CD24- 细胞表面表达来分离 BCSC。 MTT试验用于评估CSE对MDA-MB-231和BCSC的细胞毒性作用。此外，使用流式细胞术检测细胞周期分布、凋亡、ROS水平和CD44/CD24水平。使用 qRT-PCR 评估了干性标记 NANOG、SOX-2、OCT-4 和 c-MYC 的基因表达。我们发现 MDA-MB-231 包含 80% 的 BCSC 群体，CSE 显示出更有效的效果对 BCSC 的细胞毒性高于 MDA-MB-231。 CSE 通过增加 ROS 水平引起 MDA-MB-231 和 BCSC 细胞凋亡。此外，CSE 显着降低了 BCSC 中 MDA-MB-231 干性标记 CD44 /CD24- 和细胞多能标记的 mRNA 水平。CSE 可能降低 BCSC 干性，这可能是通过 ROS 水平升高和 ROS 水平降低介导的。干性转录的表达水平。版权所有：© 2023 Research in Pharmaceutical Sciences。

Breast cancer stem cells (BCSCs) as a kind of tumor cells are able to regenerate themselves, leading to apoptosis resistance and cancer relapse. It was reported that BCSCs contain lower levels of reactive oxygen species (ROS) associated with stemness capability. Caesalpinia sappan has been proposed for its chemopreventive potency against several cancer cells. This study aimed to evaluate the effects of Caesalpinia sappan extract (CSE) on cytotoxicity, apoptosis induction, ROS generation, and stemness markers of MDA-MB-231 and its BCSCs.Caesalpinia sappan was extracted under maceration with methanol. Magnetic-activated cell sorting was used to isolate BCSCs based on CD44+ and CD24- cell surface expression. The MTT test was used to assess the cytotoxic effects of CSE on MDA-MB-231 and BCSCs. Moreover, flow cytometry was used to examine the cell cycle distribution, apoptosis, ROS level, and CD44/CD24 level. Using qRT-PCR, the gene expression of the stemness markers NANOG, SOX-2, OCT-4, and c-MYC was assessed.We found that MDA-MB-231 contains 80% of the BCSCs population, and CSE showed more potent cytotoxicity toward BCSCs than MDA-MB-231. CSE caused apoptosis in MDA-MB-231 and BCSCs cells by increasing the level of ROS. Furthermore, CSE significantly reduced the MDA-MB-231 stemness marker CD44+/CD24- and the mRNA levels of pluripotent markers of cells in BCSCs.CSE potentially reduces BCSCs stemness, which may be mediated by the elevation of the ROS levels and reduction of the expression levels of stemness transcription.Copyright: © 2023 Research in Pharmaceutical Sciences.