我们提出了一种调整荧光分子结构的新策略，以实现 NIR-II 窗口尾部的发射。 YN 良好的光谱特性和低细胞毒性使其成为生物成像的强大工具。值得注意的是，YN-4的亮度是YN-3的2.5倍，是IR-783的6倍，是ICG的5倍。这种增强的亮度使得能够对小鼠胸腔和腹腔、肿瘤脉管系统进行高分辨率成像，并使用 YN-4 实时监测胃肠运动。此外，将葡萄糖共价接枝到 YN-Glu 支架上显着提高了肿瘤靶向能力并促进了葡萄糖代谢的跟踪。这项工作旨在将荧光分子成像的应用扩展到 NIR-IIa 窗口之外。版权所有 © 2024 Elsevier B.V. 保留所有权利。

We propose a novel strategy for tailoring the structure of fluorescent molecules to achieve emission at the tail end of the NIR-II window. The favorable spectroscopic properties and low cytotoxicity of YNs make them powerful tools for bioimaging. Notably, YN-4 exhibits a brightness 2.5 times greater than YN-3, 6 times that of IR-783, and 5 times that of ICG. This enhanced brightness enabled high-resolution imaging of mouse thoracic and abdominal cavities, tumor vasculature, and real-time monitoring of gastrointestinal motility using YN-4. Furthermore, covalent grafting of glucose onto the YN-Glu scaffold significantly improved tumor-targeting capability and facilitated tracking of glucose metabolism. This work aims to extend the application of fluorescent molecule imaging beyond the NIR-IIa window.Copyright © 2024 Elsevier B.V. All rights reserved.