目前的喷雾式水凝胶面膜缺乏对大面积伤口的逐步保护、清洁和滋养，导致疤痕愈合延迟。在这里，我们开发了一种可喷雾仿生双伤口面膜（BDM），具有快速自动定相和分层编程功能，可实现无疤痕伤口愈合。 BDM 包括作为顶层的疏水性聚（丙交酯-共-丙二醇-共-丙交酯）二甲基丙烯酸酯（PLD）和作为底层的亲水性甲基丙烯酸明胶（GelMA）水凝胶，能够快速自相化为双层结构。光交联后，BDM 快速固化，具有牢固的界面结合、牢固的组织粘附力和优异的关节适应性。实施后，底部的 GelMA 层可以立即释放钙离子以实现快速止血，而顶部的 PLD 层可以保持湿润、透气和无菌的环境。这些特性协同抑制炎性肿瘤坏死因子-α 通路，同时协调环磷酸鸟苷/蛋白激酶 G-Wnt/钙离子信号通路以滋养血管生成。总的来说，我们的 BDM 具有双层结构的自我调节结构，可以分层编程愈合进程，具有无疤痕伤口愈合的变革潜力。

Current sprayable hydrogel masks lack the stepwise protection, cleansing, and nourishment of extensive wounds, leading to delayed healing with scarring. Here, we develop a sprayable biomimetic double wound mask (BDM) with rapid autophasing and hierarchical programming for scarless wound healing. The BDMs comprise hydrophobic poly (lactide-co-propylene glycol-co-lactide) dimethacrylate (PLD) as top layer and hydrophilic gelatin methacrylate (GelMA) hydrogel as bottom layer, enabling swift autophasing into bilayered structure. After photocrosslinking, BDMs rapidly solidify with strong interfacial bonding, robust tissue adhesion, and excellent joint adaptiveness. Upon implementation, the bottom GelMA layer could immediately release calcium ion for rapid hemostasis, while the top PLD layer could maintain a moist, breathable, and sterile environment. These traits synergistically suppress the inflammatory tumor necrosis factor-α pathway while coordinating the cyclic guanosine monophosphate/protein kinase G-Wnt/calcium ion signaling pathways to nourish angiogenesis. Collectively, our BDMs with self-regulated construction of bilayered structure could hierarchically program the healing progression with transformative potential for scarless wound healing.