哮喘的特征是肺细胞浸润和气道高反应性。短期压力可减少气道炎症。因此，在本研究中，我们检测了反复注射生理盐水治疗引起的短期应激对大鼠肺部过敏性炎症反应的影响。成年雄性大鼠分为三组：Naïve组（未致敏、激发、或治疗的大鼠）、对照组（用卵清蛋白（OVA）致敏以诱导肺部炎症的大鼠）和盐水组（在OVA致敏前用盐水治疗大鼠五天）。在使用雾化 OVA 加强接种一周后进行吸入挑战。第18天，评估盐水注射对支气管肺泡灌洗液(BAL)、股骨髓灌洗液(FML)和血液中白细胞总数和分类白细胞的影响。还测量了肺外植体中粘液、血清皮质酮、胶原蛋白、细胞因子和去甲肾上腺素水平的百分比。OVA致敏增加了循环白细胞及其向肺的迁移，从而减少了骨髓白细胞。重复注射盐水可减少对照组 BAL 和血液中的白细胞数量，从而防止这种迁移。对照组的细胞因子白细胞介素 4 (IL-4) 高于初始组和盐水组；对照组和盐水组的细胞因子白细胞介素 6 (IL-6)、白细胞介素 10 (IL-10) 和肿瘤坏死因子 α (TNFα) 高于初始组；盐水组中的干扰素γ（IFNγ）高于初始组和对照组；在用 OVA 致敏的动物中，去甲肾上腺素增加，并且仅在盐水组中相对于未经处理的组更高。这些结果表明，短期压力可能有助于特定治疗的抗过敏性气道炎症效果。© 2024 作者。

Asthma is characterized by pulmonary cell infiltration and hyper-responsiveness of the airways. Short-term stress reduces airway inflammation. Thus, in the present study, we examined the effects of short-term stress induced by repeated treatment with saline injections on the pulmonary allergic inflammatory response in rats.Adult male rats were divided into three groups: Naïve group (non-sensitized, challenged, or treated rats), Control group (rats sensitized with ovalbumin (OVA) to induce lung inflammation), and Saline group (rats treated for five days with saline before OVA sensitization). Inhalation challenges were performed one week after the booster with aerosolized OVA. On day 18, the effect of saline injections on total and differential leukocytes in bronchoalveolar lavage (BAL), femoral marrow lavage (FML), and blood was evaluated. The percentage of mucus, serum corticosterone, collagen, cytokines in lung explants, and norepinephrine levels were also measured.OVA sensitization increased the circulating leukocytes and their migration to the lung, decreasing the bone marrow leukocytes. The repeated saline injections prevented this migration by decreasing the number of leukocytes in BAL and blood in the control group. Cytokine Interleukin-4 (IL-4) was higher in the control group than in the naive and saline groups; cytokines Interleukin-6 (IL-6), Interleukin-10 (IL-10), and tumor necrosis factor-alpha (TNFα) were higher in the control and saline groups than in the naïve group; Interferon gamma (IFNγ) was higher in the saline group than in the naive and control groups; norepinephrine increased in animals sensitized with OVA and was higher only in the saline group relative to the naïve group.These results suggest that short-term stress could contribute to the anti-allergic airway inflammation effects of a given treatment.© 2024 The Authors.