白细胞介素1β(IL1β)、IL6、肿瘤坏死因子-α(TNF-α)等炎性细胞因子可抑制成骨细胞分化并诱导成骨细胞凋亡。 PANoptosis 是一种新发现的程序性细胞死亡 (PCD) 类型，可能受到长链非编码 RNA (lncRNA) 的影响，而长链非编码 RNA (lncRNA) 在调节炎症方面发挥着重要作用。然而，lncRNA 在成骨分化过程中炎症和 PANoptosis 中的潜在作用仍不清楚。本研究旨在探讨lncRNA在成骨分化过程中炎症和细胞凋亡中的调控功能。利用高通量测序技术鉴定炎症条件下参与成骨细胞分化的差异表达基因。从测序数据和基因表达综合（GEO）数据库中鉴定出两种与成骨分化过程中的炎症和 PANoptosis 相关的 lncRNA。使用不同的生物信息学方法分析它们的功能，从而构建 lncRNA-miRNA-mRNA 网络。其中，lncRNA (MIR17HG) 显示与 PANoptosis 高度相关。采用文献计量学方法收集有关PANoptosis的文献数据，并推断其组成部分。 PCR和Western Blotting实验证实lncRNA MIR17HG与炎症期间成骨细胞的PANoptosis相关。我们的数据表明TNF-α诱导的成骨分化抑制和MC3T3-E1成骨细胞的PANoptosis与MIR17HG相关。这些发现强调了 MIR17HG 在炎症、全凋亡和成骨分化之间相互作用中的关键作用，提示了涉及骨形成和炎症反应受损疾病的潜在治疗靶点。© 2024。作者。

Inflammatory cytokines such as Interleukin 1β(IL1β), IL6,Tumor Necrosis Factor-α (TNF-α) can inhibit osteoblast differentiation and induce osteoblast apoptosis. PANoptosis, a newly identified type of programmed cell death (PCD), may be influenced by long noncoding RNA (lncRNAs) which play important roles in regulating inflammation. However, the potential role of lncRNAs in inflammation and PANoptosis during osteogenic differentiation remains unclear. This study aimed to investigate the regulatory functions of lncRNAs in inflammation and apoptosis during osteogenic differentiation.High-throughput sequencing was used to identify differentially expressed genes involved in osteoblast differentiation under inflammatory conditions. Two lncRNAs associated with inflammation and PANoptosis during osteogenic differentiation were identified from sequencing data and Gene Expression Omnibus (GEO) databases. Their functionalities were analyzed using diverse bioinformatics methodologies, resulting in the construction of the lncRNA-miRNA-mRNA network. Among these, lncRNA (MIR17HG) showed a high correlation with PANoptosis. Bibliometric methods were employed to collect literature data on PANoptosis, and its components were inferred. PCR and Western Blotting experiments confirmed that lncRNA MIR17HG is related to PANoptosis in osteoblasts during inflammation.Our data suggest that TNF-α-induced inhibition of osteogenic differentiation and PANoptosis in MC3T3-E1 osteoblasts is associated with MIR17HG. These findings highlight the critical role of MIR17HG in the interplay between inflammation, PANoptosis, and osteogenic differentiation, suggesting potential therapeutic targets for conditions involving impaired bone formation and inflammatory responses.© 2024. The Author(s).