由于原代脂肪细胞的培养具有挑战性，我们对脂肪细胞如何影响代谢信号、免疫功能和癌症进展的理解仍然有限。在这里，我们提出了一种制造弹性体微孔的协议，用于胶原蛋白嵌入脂肪细胞的三维培养。我们描述了固化和功能化弹性体微孔以及分离和嵌入原代脂肪细胞的步骤。然后我们详细介绍如何培养和分析脂肪细胞胶原蛋白凝胶。该协议提供了广泛的应用，以提高我们对健康和疾病中脂肪细胞生物学的理解。版权所有 © 2024 作者。由爱思唯尔公司出版。保留所有权利。

Our understanding of how adipocytes influence metabolic signaling, immune function, and cancer progression remains limited as the culture of primary adipocytes is challenging. Here, we present a protocol to fabricate elastomer microwells for three-dimensional culture of collagen-embedded adipocytes. We describe steps to cure and functionalize elastomer microwells and to isolate and embed primary adipocytes. We then detail how to culture and analyze adipocyte-collagen gels. This protocol provides broad applications to improve our understanding of adipocyte biology in health and disease.Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.