黄酮类化合物的药理特性已有报道，其中具有抗癌作用，但其机制尚未完全阐明。在本研究中，研究了槲皮素和白杨素对 MCF-7 和 MDA-MB-231 乳腺癌细胞的活性。用不同浓度的化合物处理24小时后测定细胞活力。其次，用固定浓度的白杨素和不同浓度的槲皮素处理细胞，预孵育1h。两种化合物均以剂量依赖性方式抑制细胞增殖。这种关联表明它们的细胞毒性有所改善，在槲皮素预孵育时表现得更明显。槲皮素和白杨素联合诱导 MDA-MB-231 细胞的细胞周期停滞在亚 G0/G1 期，改变 caspase-3 和 -8,-8 的表达，诱导晚期细胞凋亡。总而言之，我们的结果表明，两种黄酮类化合物均以剂量依赖性方式抑制细胞生长，并且槲皮素的结合改善了白杨素对细胞系的毒性作用。版权所有 © 2024 作者。由 Elsevier Masson SAS 出版。保留所有权利。

Pharmacological properties of flavonoids have been reported, with an anticancer role amongst them, however, its mechanisms are not fully elucidated. In this study, the activity of quercetin and chrysin towards MCF-7 and MDA-MB-231 breast cancer cells was investigated. Cellular viability was determined after treatment with the compounds in different concentrations for 24 h. Secondly, cells were treated with fixed concentration of chrysin and different concentrations of quercetin with preincubation for 1 h. Both compounds inhibited cellular proliferation in dose-dependent manner. The association showed improvement in their cytotoxicity, more expressively with preincubation of quercetin. Quercetin and chrysin association induced cell cycle arrest in sub-G0/G1 phase in MDA-MB-231 cells, modified the expression of caspases-3 and -8,-8, inducing late apoptosis cell death. Taken together, our results demonstrate that both flavonoids inhibited cells growth in a dose-dependent manner and the association of quercetin improved chrysin's toxic effect over the cell lines.Copyright © 2024 The Authors. Published by Elsevier Masson SAS.. All rights reserved.