研究炎症小体活动的传统方法和最新工具。
Traditional approaches and recent tools for studying inflammasome activity.
发表日期：2024 Aug 13
作者：
Cassio Luiz Coutinho Almeida-da-Silva, Aline Cristina de Abreu Moreira-Souza, David M Ojcius
来源：
CYTOKINE & GROWTH FACTOR REVIEWS
摘要：
炎性体在对感染的免疫反应、自身免疫性疾病的发展和癌症的控制中发挥着重要作用。蛋白质印迹最初于 2000 年代初用于表征炎症小体激活。从那时起，人们开发了一系列技术来表征和可视化细胞、组织和动物中炎症小体的激活。这篇综述文章描述了研究人员在炎症小体领域最常用的技术，并提出组织或动物中炎症小体激活的细胞特异性表征可能很快就会被普遍报道。版权所有 © 2024。由 Elsevier B.V. 出版。
Inflammasomes play a major role in the immune response to infection, development of autoimmune disease, and control of cancer. Western blots were originally used in the early 2000s to characterize inflammasome activation. Since then, a panoply of techniques has been developed to characterize and visualize inflammasome activation in cells, tissues, and animals. This review article describes the most common techniques used by researchers in the inflammasome field and proposes that cell-specific characterization of inflammasome activation in tissues or animals may soon be commonly reported.Copyright © 2024. Published by Elsevier B.V.