骨组织工程旨在解决由创伤、感染、肿瘤和手术引起的与骨相关的问题。基于聚合物和硅酸钙生物活性材料（BM）的复合材料通常优选作为骨治疗的潜在材料。然而，该聚合物的生物活性较低，因此，当前的工作旨在通过改变Alg百分比来优化复合材料的多孔性质，制备基于BM-海藻酸钠（Alg）的复合支架。首先，使用稻壳和蛋壳作为二氧化硅和钙的前体，通过简单的沉淀方法合成BM，同时通过简单的交联方法制备BM-Alg复合材料。使用 XRD、FTIR、SEM 和 BET 技术研究了 BM-Alg 复合材料。此外，在模拟体液（SBF）中进行了体外生物活性研究，显示羟基磷灰石的形成。体外溶血研究显示溶血率低于5%。随后，使用体外 CAM 模型进行血管生成研究，该模型揭示了新血管形成的增强。 MG-63细胞用于研究生物相容性，在10 mg mL-1的浓度下表现出无毒性质。此外，体内生物相容性结果也揭示了其无毒性质。因此，BM-Alg 复合材料可作为骨组织工程应用的潜在生物相容性材料。该期刊版权所有 © 英国皇家化学学会。

Bone tissue engineering aims to address bone-related problems that arise from trauma, infection, tumors, and surgery. Polymer and calcium silicate bioactive material (BM) based composites are commonly preferred as potential materials for bone treatment. However, the polymer has low bioactivity, thus, the current work aims to prepare a composite scaffold based on BM-sodium alginate (Alg) by varying the Alg percentage to optimize the porous nature of the composite. Primarily, the BM was synthesized by a simple precipitation method using rice husk and eggshell as the precursors of silica and calcium, while the BM-Alg composite was prepared by a facile cross-linking approach. The BM-Alg composite was studied using XRD, FTIR, SEM, and BET techniques. Further, an in vitro bioactivity study was performed in simulated body fluid (SBF) which shows hydroxyapatite formation. The in vitro haemolysis study displayed less than 5% haemolysis. Subsequently, the angiogenesis study was carried out using the ex ovo CAM model which reveals enhanced neovascularization. The MG-63 cells were used to study the biocompatibility, and they displayed a non-toxic nature at a concentration of 10 mg mL-1. Further, the in vivo biocompatibility results also reveal its non-toxic nature. Thus, the BM-Alg composite acts as a potential biocompatible material for bone tissue engineering applications.This journal is © The Royal Society of Chemistry.