鱿鱼（Sepioteuthis Lessoniana）是一种卓越的渔业产品，许多国家都出口该产品用于工业生产或人类消费。本研究的重点是从乌贼墨（SI）中合成银纳米颗粒（AgNP）及其广泛的应用。通过紫外-可见光谱、FTIR、XRD、SEM、EDX、DLS 和 zeta 电位分析证实了纳米颗粒的形成。结果显示在 407 nm 处有强吸收峰，存在各种官能团，晶体尺寸为 17.56 nm 的纳米晶体结构，平均尺寸为 76 nm 的球形颗粒，并且存在最高质量百分比分别为Ag和均匀分散的颗粒。通过抗菌、抗氧化、抗癌和毒性研究，分析了合成的鱿鱼墨银纳米颗粒的生物活性。染料降解测定也作为不同工业染料废水处理的一种手段进行了分析。在浓度为 100 μg/ml 时，对大肠杆菌的抗菌活性表现出最高的抑制范围，为 24 mm，其次是其他测试菌株。一氧化氮自由基清除测定显示，浓度为 100 μg/ml 时抗氧化活性最高 (92%)。 SI-AgNP 对 MDA-MB-231 乳腺癌细胞系的细胞毒性能力显示 IC50 值为 4.52 μg/ml。毒性研究显示，活性具有剂量和时间依赖性，24 小时和 48 小时的 LC50 值分别为 5.090 和 3.303 mg/ml。 SI-AgNPs对染料的成功降解归因于以Ag为催化剂和SI为催化载体的电子中继系统的协同作用。这些发现表明 SI-AgNP 是一种新型潜在产品，应进一步研究以改善其药理学、生物医学和环境应用。© 2024。作者获得 Springer Science Business Media, LLC 的独家许可，该公司是施普林格自然。

The squid, Sepioteuthis lessoniana, is a remarkable fishery product which is exported by many nations for use in industrial production or human consumption. This study focused on the synthesis of silver nanoparticles (AgNPs) from squid ink (SI) and its wide range of applications. The formation of the nanoparticles was confirmed through UV-Visible spectroscopy, FTIR, XRD, SEM with EDX, DLS, and zeta potential analysis. The results showed a strong absorbance peak at 407 nm, the presence of various functional groups, a nanocrystalline structure with a crystalline size of 17.56 nm, spherical-shaped particles with an average size of 76 nm, and the presence of the highest % mass of Ag and uniformly dispersed particles, respectively. The bioactivity of the synthesized squid ink silver nanoparticles was analyzed through antibacterial, antioxidant, anticancer, and toxicity studies. The dye degradation assay was also analyzed as a means of wastewater treatment for different industrial dyes. The antibacterial activity showed the highest zone of inhibition of 24 mm at a concentration of 100 μg/ml against Escherichia coli, followed by other tested strains. The nitric oxide radical scavenging assay showed the highest antioxidant activity (92%) at a concentration of 100 μg/ml. The cytotoxic ability of SI-AgNPs against the MDA-MB-231 breast cancer cell line revealed an IC50 value of 4.52 μg/ml. The toxicity study revealed a dose and time-dependent activity with the LC50 value of 5.090 and 3.303 mg/ml for 24 and 48 h, respectively. The successful degradation of dyes by SI-AgNPs is attributed to the cooperative action of the electron relay system with Ag as a catalyst and SI as a catalytic support. These findings indicate that SI-AgNPs are a novel potential product that should be further studied to improve its pharmacological, biomedical, and environmental applications.© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.