结直肠癌（CRC）是第四大常见恶性肿瘤死亡原因。迫切需要开发新型、更有效的药物来治疗结直肠癌。生姜因其类黄酮和酚类而被认为具有抗癌特性。本研究采用索氏提取 (SOXT) 和浸渍 (MACR) 技术，旨在评估生姜胶囊中槲皮素、没食子酸、芦丁、柚皮苷和咖啡酸的含量。使用高效液相色谱（HPLC）/紫外线进行分离和定量。还进行了生姜胶囊对 CRC 细胞系 HT-29 的体外毒性评估，以评估该补充剂的抗癌活​​性。利用 HT-29 (HTB-38) 结直肠腺癌细胞系来研究 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴化物的抗增殖作用。 200 和 100μg 剂量的生姜草药补充剂提取物通过 MACR 对 HT-29 CRC 细胞具有强烈的细胞毒性作用 (IC50<50μg/mL)。该提取物与 SOXT 提取物相当，后者的 IC50 小于 50μg/mL。研究了生姜草药补充剂配方对 CRC 细胞系的抗癌作用，并且从 MACR 和 SOXT 提取程序获得的结果值得注意。根据 HPLC 数据，槲皮素含量是所有提取物中最高的。© 2024 John Wiley

Colorectal cancer (CRC) is the fourth most common cause of malignant tumor death. The development of novel, more effective drugs is desperately needed to treat CRC. Zingiber officinale is believed to possess anticancer properties due to its flavonoids and phenols. Using Soxhlet (SOXT) and maceration (MACR) techniques, the present study aimed to evaluate the amounts of quercetin, gallic acid, rutin, naringin, and caffeic acid in ginger capsules of Z. officinale. High-performance liquid chromatography (HPLC)/ultraviolet was used for separation and quantitation. In vitro toxicity evaluation of ginger capsules on the CRC cell line HT-29 was also conducted to assess the anticancer activity of the supplement. The cell line HT-29 (HTB-38) colorectal adenocarcinoma was utilized for the antiproliferative effect of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide. Ginger herbal supplement extract at dosages of 200 and 100 μg had strong cytotoxic effects (IC50 < 50 μg/mL) on HT-29 CRC cells via MACR. This extract is comparable to the SOXT extract, which has an IC50 of less than 50 μg/mL. The anticancer effect of ginger herbal supplement formulations against CRC lines was investigated, and the results obtained from both the MACR and SOXT extraction procedures were noteworthy. The quercetin content was the highest of all the extracts according to the HPLC data.© 2024 John Wiley & Sons Ltd.