硫酸乙酰肝素 2-O-磺基转移酶 1 (HS2ST1) 催化硫酸乙酰肝素蛋白聚糖中葡萄糖醛酸残基的硫酸化，使这些蛋白聚糖能够与肿瘤微环境中的众多配体相互作用。然而，HS2ST1表达在癌症中的预后作用仍不清楚。本研究调查了HS2ST1表达水平及其在各种癌症类型中的预后意义，证明了HS2ST1表达在肝细胞癌（HCC）患者中的预后价值，并鉴定了与HS2ST1表达相关的分子特征使用基因表达谱交互分析 (GEPIA) 门户对来自癌症基因组图谱 (TCGA) 数据集的 HS2ST1 表达和患者生存数据进行分析。我们从 TCGA 以及日本和法国国际癌症基因组联盟 (ICGC) 数据库中获取了 HCC 患者的基因表达和临床病理信息，并进行了生存分析。我们还检查了与 HS2ST1 表达相关的相关蛋白网络、差异表达基因、基因集富集和肿瘤免疫微环境特征。与正常组织相比，HS2ST1 在八种肿瘤类型中表现出较高的表达，并且与包括 HCC 在内的五种肿瘤的不良预后相关。在两个 ICGC HCC 队列中，HS2ST1 状态与不良预后相关。 HCC 肿瘤中 HS2ST1 表达升高与参与细胞周期进展、蛋白质分泌和 mTORC1 信号传导的信号通路相关。此外，HS2ST1 表达水平与肿瘤微环境中的免疫细胞浸润呈负相关。我们的研究阐明了 HCC 患者中 HS2ST1 表达的预后意义，并深入了解 HS2ST1 在信号通路和肿瘤微环境中的潜在作用。© 2024。作者。

Heparan sulfate 2-O-sulfotransferase 1 (HS2ST1) catalyzes the sulfation of glucuronic acid residues in heparan sulfate proteoglycans, enabling these proteoglycans to interact with numerous ligands within tumor microenvironments. However, the prognostic role of HS2ST1 expression in cancer remains unclear.This investigated HS2ST1 expression levels and their prognostic significance in various cancer types, demonstrated the prognostic value of HS2ST1 expression in hepatocellular carcinoma (HCC) patients, and identified molecular signatures associated with HS2ST1 expression.HS2ST1 expression and patient survival data from The Cancer Genome Atlas (TCGA) datasets were analyzed using the Gene Expression Profiling Interactive Analysis (GEPIA) portal. We obtained gene expression and clinicopathological information on HCC patients from the TCGA and the Japan and France International Cancer Genome Consortium (ICGC) databases and performed survival analyses. We also examined relevant protein networks, differentially expressed genes, gene set enrichments, and tumor immune microenvironment features associated with HS2ST1 expression.HS2ST1 exhibited higher expression in eight tumor types compared with normal tissues and was associated with poor prognoses in five tumors, including HCC. HS2ST1 status correlated with poor prognosis in two ICGC HCC cohorts. Elevated HS2ST1 expression in HCC tumors was associated with signaling pathways involved in cell cycle progression, protein secretion, and mTORC1 signaling. Moreover, HS2ST1 expression levels were inversely correlated with immune cell infiltration in the tumor microenvironment.Our study elucidates the prognostic significance of HS2ST1 expression in HCC patients and provides insights into the potential roles of HS2ST1 in signaling pathways and the tumor microenvironment.© 2024. The Author(s).