瞬时受体电位（TRP）通道参与肿瘤的发生和进展。然而，它们在结直肠癌（CRC）中的作用仍不清楚，本研究旨在探讨TRP相关基因在CRC中的作用。数据来自癌症基因组图谱（TCGA）数据库，并对GSE14333和GSE14333进行分析。 GSE38832 数据集用于评估预后并标记 TRP 相关基因 (TRG)。随后，进行聚类分析和免疫浸润分析来探索相关的TRG。利用人结肠癌细胞对关键TRGs基因和蛋白表达进行体外验证。与正常组织相比，CRC中8个TRGs显着上调，11个TRGs下调。 TRPA1被确定为保护性预后因素，而TRPM5（HR = 1.349）、TRPV4（HR = 1.289）和TRPV3（HR = 1.442）被确定为预后危险因素。受试者工作特征 (ROC) 曲线和 Kaplan-Meier (KM) 分析得出了相似的结果。此外，TRPA1 的较低表达以及 TRPV4 和 TRPM5 的较高表达与患者预后呈负相关，实验验证证实了 CRC 细胞系中 TRPA1 的表达不足和 TRPV4 和 TRPM5 的过度表达。本研究确定了 CRC 中 TRP 通道相关的预后，提供了一种对 CRC 预后进行分层的新颖方法。© 2024。作者。

Transient receptor potential (TRP) channels are involved in the development and progression of tumors. However, their role in colorectal cancer (CRC) remains unclear, and this study aims to investigate the role of TRP-related genes in CRC.Data was obtained from The Cancer Genome Atlas (TCGA) database, and analyses were conducted on the GSE14333 and GSE38832 datasets to assess the prognosis and mark TRP-related genes (TRGs). Subsequently, clustering analysis and immune infiltration analysis were performed to explore the relevant TRGs. In vitro validation of key TRGs' gene and protein expression was conducted using human colon cancer cells.Compared to normal tissues, 8 TRGs were significantly upregulated in CRC, while 11 were downregulated. TRPA1 was identified as a protective prognostic factor, whereas TRPM5 (HR = 1.349), TRPV4 (HR = 1.289), and TRPV3 (HR = 1.442) were identified as prognostic risk factors. Receiver operating characteristic (ROC) curves and Kaplan-Meier (KM) analyses yielded similar results. Additionally, lower expression of TRPA1 and higher expression of TRPV4 and TRPM5 were negatively correlated with patient prognosis, and experimental validation confirmed the underexpression of TRPA1 and overexpression of TRPV4 and TRPM5 in CRC cell lines.This study identifies a TRP channel-related prognosis in CRC, providing a novel approach to stratifying CRC prognosis.© 2024. The Author(s).