嘌呤能配体门控离子通道7受体（P2X7R）是一种ATP门控离子通道，可传递细胞外信号并诱导相应的生物效应，瞬时受体电位香草酸1型（TRPV1）是一种非选择性阳离子通道，维持正常生理功能;大量研究表明P2X7R和TRPV1与炎症反应相关。研究了高糖高游离脂肪(HGHF)环境下卫星胶质细胞(SGC)中P2X7R敲低对神经元TRPV1表达的影响。利用 P2X7 短发夹 RNA (shRNA) 下调 SGC 中的 P2X7R，并将处理和未处理的 SGC 与神经元细胞系共培养。使用蛋白质印迹分析、RT-qPCR、免疫荧光和酶联免疫吸附测定来测量 SGC 和神经元中炎症因子和信号通路的表达水平。结果表明，P2X7 shRNA 降低了 DRG 神经元周围 SGC 中 P2X7R 蛋白和 mRNA 的表达水平，并通过 Ca2 /p38 MAPK/NF-κB 途径下调肿瘤坏死因子-α 和白细胞介素-1β 的释放。此外，P2X7R 的下调可能通过 Ca2 /PKC-ɛ/p38 MAPK 途径降低神经元中 TRPV1 的表达。在 HGHF 环境中减少 SCG 中 P2X7R 的表达可以通过 Ca2 /PKC-ɛ/p38 MAPK 途径降低神经元 TRPV1 的表达。 © 2024 Société Française des Microscopies 和 Société de Biologie Cellulaire de France。约翰·威利出版

The purinergic ligand-gated ion channel 7 receptor (P2X7R) is an ATP-gated ion channel that transmits extracellular signals and induces corresponding biological effects, transient receptor potential vanilloid type 1 (TRPV1) is a non-selective cation channel that maintains normal physiological functions; numerous studies showed that P2X7R and TRPV1 are associated with inflammatory reactions.The effect of P2X7R knockdown in satellite glial cells (SGCs) on neuronal TRPV1 expression under high glucose and high free fat (HGHF) environment was investigated. P2X7 short hairpin RNA (shRNA) was utilized to downregulate P2X7R in SGCs, and treated and untreated SGCs were co-cultured with neuronal cell lines. The expression levels of inflammatory factors and signaling pathways in SGCs and neurons were measured using Western blot analysis, RT-qPCR, immunofluorescence, and enzyme-linked immunosorbent assays. Results suggested that P2X7 shRNA reduced the expression levels of P2X7R protein and mRNA in SGCs surrounding DRG neurons and downregulated the release of tumor necrosis factor-alpha and interleukin-1 beta via the Ca2+/p38 MAPK/NF-κB pathway. Additionally, the downregulation of P2X7R might decrease TRPV1 expression in neurons via the Ca2+/PKC-ɛ/p38 MAPK pathway.Reducing P2X7R expression in SCGs in an HGHF environment could decrease neuronal TRPV1 expression via the Ca2+/PKC-ɛ/p38 MAPK pathway.© 2024 Société Française des Microscopies and Société de Biologie Cellulaire de France. Published by John Wiley & Sons Ltd.