铝佐剂仍然是最常用的疫苗佐剂。然而，铝佐剂在触发体液免疫方面相当有效，但在激活细胞免疫方面通常表现出有限的能力。本文通过在铝佐剂的制备过程中添加锰离子，得到锰修饰的铝(Mn-Al)佐剂，其能够有效刺激体液和细胞免疫反应。这种Mn-Al佐剂可以增强抗原吸附并促进树突状细胞(DC)内化抗原。随后，释放的Mn2可以激活干扰素基因途径的环磷酸鸟苷-磷酸腺苷合酶刺激剂，进一步促进DC活化。当与模型抗原卵清蛋白（OVA）结合时，锰铝佐剂疫苗可在体内诱导高水平的抗原特异性抗体滴度和高比例的抗原特异性细胞毒性T细胞。此外，与高剂量的铝佐剂相比，锰铝佐剂疫苗引发更强的抗原特异性体液和细胞免疫反应。此外，在Mn-Al佐剂存在下用OVA免疫小鼠显着抑制了B16-OVA肿瘤的生长。此外，当与人乳头瘤病毒抗原配制时，锰铝佐剂疫苗表现出比铝佐剂疫苗更好的体内疫苗接种性能。因此，锰改性铝佐剂可能成为一种新型疫苗佐剂，具有替代传统铝佐剂的潜力。© 2024 Wiley‐VCH GmbH。

Aluminum adjuvants remain the most commonly used vaccine adjuvants. Being rather effective in triggering humoral immunity, however, aluminum adjuvants usually show limited abilities in activating cellular immunities. Herein, by adding manganese ions during the preparation of aluminum adjuvant, a manganese-modified aluminum (Mn-Al) adjuvant is obtained, which can effectively stimulate both humoral and cellular immune responses. Such Mn-Al adjuvant can enhance antigen adsorption and promote antigen internalization by dendritic cells (DCs). Subsequently, the released Mn2+ can activate the cyclic guanosine monophosphate-adenosine monophosphate synthase-stimulator of interferon genes pathway to further promote DC activation. When combines with the model antigen ovalbumin (OVA), the Mn-Al-adjuvantes vaccine can induce high levels of antigen-specific antibody titers and high proportions of antigen-specific cytotoxic T cells in vivo. Moreover, the Mn-Al-adjuvanted vaccine elicited stronger antigen-specific humoral and cellular immune responses than high-dose of the aluminum-based adjuvant. Additionally, immunization of mice with OVA in the presence of the Mn-Al adjuvant significantly inhibited the growth of B16-OVA tumors. Furthermore, when formulated with human papillomavirus antigens, Mn-Al-adjuvanted vaccines show better in vivo vaccination performance than aluminum-adjuvanted vaccines. Therefore, the manganese-modified aluminum adjuvant may thus become a new vaccine adjuvant with the potential to replace conventional aluminum adjuvants.© 2024 Wiley‐VCH GmbH.