天然药物在治疗骨疾病方面的治疗潜力是众所周知的。配方或分子结构的修改可以增强其功效。姜酚是一种源自姜根（Zingiber officinale）的成骨活性化合物，可以形成金属离子复合物。锌 (Zn) 是一种对抗细菌感染并促进成骨细胞增殖的微量元素，可以与姜辣素复合形成 G-Zn 2 复合物。本研究研究了负载 G-Zn 2 复合物的多孔 3D 打印 (3DP) 磷酸钙 (CaP) 支架，用于药物释放和细胞相互作用。支架上涂有聚己内酯（PCL）以控制药物释放。扩散介导的动力学导致 G-Zn 2 复合物在 6 周内释放 50%。 G-Zn 2 复合物表现出针对 MG-63 骨肉瘤细胞的细胞毒性，通过支架上凋亡小体的形成和破裂的细胞形态表明。与未经处理的支架相比，G-Zn 2 PCL 涂层支架的成骨细胞活力增加了 1.2±0.1 倍，碱性磷酸酶增加了 11.6±0.5%。经过处理的支架还表现出针对金黄色葡萄球菌的细菌定植减少，凸显了 G-Zn 2 复合物的抗菌潜力。具有 G-Zn 2 复合物的功能化 3DP CaP 支架在低承载应用中显示出增强骨再生和预防感染的巨大潜力。© 2024。控释协会。

The therapeutic potential of natural medicines in treating bone disorders is well-established. Modifications in formulation or molecular structure can enhance their efficacy. Gingerol, an osteogenic active compound derived from ginger roots (Zingiber officinale), can form metal ion complexes. Zinc (Zn), a trace element that combats bacterial infections and promotes osteoblast proliferation, can be complexed with gingerol to form a G-Zn+2 complex. This study investigates a porous 3D-printed (3DP) calcium phosphate (CaP) scaffold loaded with the G-Zn+2 complex for drug release and cellular interactions. The scaffold is coated with polycaprolactone (PCL) to control the drug release. Diffusion-mediated kinetics results in 50% release of the G-Zn+2 complex over 6 weeks. The G-Zn+2 complex demonstrates cytotoxicity against MG-63 osteosarcoma cells, indicated by the formation of apoptotic bodies and ruptured cell morphology on the scaffolds. G-Zn+2 PCL-coated scaffolds show a 1.2 ± 0.1-fold increase in osteoblast cell viability, and an 11.6 ± 0.5% increase in  alkaline phosphatase compared to untreated scaffolds. Treated scaffolds also exhibit reduced bacterial colonization against Staphylococcus aureus bacteria, highlighting the antibacterial potential of the G-Zn+2 complex. The functionalized 3DP CaP scaffold with the G-Zn+2 complex shows significant potential for enhancing bone regeneration and preventing infections in low-load-bearing applications.© 2024. Controlled Release Society.