由于其浸润性，神经胶质瘤仍然是脑肿瘤的治疗难题。手术期间准确识别肿瘤边界对于成功切除至关重要。本研究介绍了一种创新的术中可视化方法，利用手术荧光显微镜精确定位肿瘤细胞的扩散。这里的重点是开发一种新型造影剂（IR-Glint），用于术中可视化包含红外标记的 Glint 适体的人类神经胶质瘤。使用流式细胞术和显微镜对原代细胞培养物评估 IR-Glint 的特异性。在小鼠和兔子模型上研究体内有效性，采用人脑胶质瘤的原位异种移植和各种成像技术，包括 PET/CT、体内荧光可视化、共焦激光扫描和手术显微镜。这些实验验证了 IR-Glint 使用红外成像术中可视化神经胶质瘤的潜力。 IR-Glint 可穿透血脑屏障，可用于静脉内和表面应用，从而使肿瘤清晰可见。直接应用于大脑的表面减少了所需的剂量并减轻了对患者的潜在毒性作用。该研究表明红外染料标记的适体在脑部手术期间准确可视化神经胶质瘤的潜力。这种新型适体辅助荧光引导手术（AptaFGS）可能为神经外科领域的未来发展铺平道路。

Gliomas remain challenging brain tumors to treat due to their infiltrative nature. Accurately identifying tumor boundaries during surgery is crucial for successful resection. This study introduces an innovative intraoperative visualization method utilizing surgical fluorescence microscopy to precisely locate tumor cell dissemination. Here, the focus is on the development of a novel contrasting agent (IR-Glint) for intraoperative visualization of human glial tumors comprising infrared-labeled Glint aptamers. The specificity of IR-Glint is assessed using flow cytometry and microscopy on primary cell cultures. In vivo effectiveness is studied on mouse and rabbit models, employing orthotopic xenotransplantation of human brain gliomas with various imaging techniques, including PET/CT, in vivo fluorescence visualization, confocal laser scanning, and surgical microscopy. The experiments validate the potential of IR-Glint for the intraoperative visualization of gliomas using infrared imaging. IR-Glint penetrates the blood-brain barrier and can be used for both intravenous and surface applications, allowing clear visualization of the tumor. The surface application directly to the brain reduces the dosage required and mitigates potential toxic effects on the patient. The research shows the potential of infrared dye-labeled aptamers for accurately visualizing glial tumors during brain surgery. This novel aptamer-assisted fluorescence-guided surgery (AptaFGS) may pave the way for future advancements in the field of neurosurgery.