基于Ti3C2Tx/AuNP和Ti3C2Tx/HKUST-1/TB复合材料的标记夹心型电化学免疫传感器用于早期肝癌检测。
Labeled sandwich-type electrochemical immunosensor based on Ti3C2Tx/AuNP and Ti3C2Tx/HKUST-1/TB composites for early liver cancer detection.
发表日期：2024 Aug 27
作者：
Haotian Wu, Gang Zhang, Xiaozhan Yang
来源：
BIOSENSORS & BIOELECTRONICS
摘要：
提出了一种新型夹心型电化学免疫传感器，用于检测人血清中肝癌标志物甲胎蛋白（AFP）。首先采用刻蚀和超声剥离制备二维MXene材料Ti3C2TX，然后用Ti3C2TX还原氯金酸形成Ti3C2TX/AuNP复合材料，并在玻碳电极表面进行修饰，形成探针型传感器。 Ti3C2TX/AuNPs为AFP捕获抗体(Ab1)提供了大量的结合位点，增加了电化学反应活性位点。采用溶剂热法制备了Ti3C2TX/铜金属有机框架HKUST-1复合材料，并与甲苯胺蓝（TB）和AFP检测抗体（Ab2）结合形成标记夹心型电化学免疫传感器。该传感器实现了0.1~100 ng/mL的AFP痕量检测，检测限为0.073 pg/mL，具有良好的选择性、稳定性和重现性。该传感器在临床样本中表现良好，具有良好的临床应用潜力。© 2024。作者获得 Springer-Verlag GmbH Austria（Springer Nature 旗下公司）的独家许可。
A novel sandwich-type electrochemical immunosensor for the detection of the liver cancer marker alpha-fetoprotein (AFP) in human serum is proposed. The two-dimensional MXene material Ti3C2Tx was first prepared using etching and ultrasonic stripping, and then Ti3C2Tx was used to reduce chloroauric acid to form Ti3C2Tx/AuNP composites which were modified on the surface of the glassy carbon electrodes to form probe-type sensors. The Ti3C2Tx/AuNPs provide a large number of binding sites for the AFP capture antibody (Ab1) and increase the electrochemical reaction active site. The Ti3C2Tx/copper metal-organic frameworks HKUST-1 composite was also prepared by solvothermal method and combined with toluidine blue (TB) and AFP detection antibody (Ab2) to form a labeled sandwich-type electrochemical immunosensor. The sensor achieved trace detection of AFP from 0.1 to 100 ng/mL with a detection limit of 0.073 pg/mL and possesses good selectivity, stability, and reproducibility. The sensor performs well in clinical samples and has good potential for clinical applications.© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.