需要癌症恶病质小鼠模型来概括恶病质患者的临床特征。在这里，我们提出了建立和评估癌症恶病质小鼠模型的方案。我们描述了准备肿瘤细胞用于接种和外科手术的步骤。在建立这些小鼠模型后，我们描述了评估癌症恶病质的基本技术，包括握力评估、组织收集和肌肉组织横截面积的计算。有关该协议的使用和执行的完整详细信息，请参阅 Liu 等人、1 Yang 等人、2 Shi 等人、3 和 Zhou 等人4。版权所有 © 2024 作者。由爱思唯尔公司出版。保留所有权利。

Cancer cachexia mouse models are needed to recapitulate the clinical features of patients with cachexia. Here, we present a protocol for the establishment and evaluation of cancer cachexia mouse models. We delineate the steps in preparing tumor cells for inoculation and surgical procedures. After the establishment of these mouse models, we describe essential techniques to assess cancer cachexia, including grip strength evaluation, tissue collection, and the calculation of cross-sectional areas of muscle tissue. For complete details on the use and execution of this protocol, please refer to Liu et al.,1 Yang et al.,2 Shi et al.,3 and Zhou et al.4.Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.