急性淋巴细胞白血病（ALL）是一种常见的血液系统癌症，在所有年龄段的人群中都有独特的发病率和预后模式。最近的分子生物学进展阐明了 ALL 复杂的分子通路，特别是 Hedgehog (Hh) 信号系统和非编码 RNA (ncRNA)。这项工作旨在通过集中研究长非编码 RNA (lncRNA) 及其与重要 Hh 通路基因的相互作用，揭示 Hh 信号传导与 ALL 之间联系的分子复杂性。为了分析 ALL 中差异表达的 lncRNA 和基因，来自使用系统生物学方法重新分析基因表达综合 (GEO)。鉴定了 Hh 信号通路相关基因，并使用 Pearson 相关分析分析了它们与差异表达的长非编码 RNA (DElncRNA) 的关系。通过识别针对 Hh 信号通路相关 mRNA 的 miRNA 构建了调控网络。在 ALL 和正常骨髓样本之间发现了 193 DEG 和 226 DElncRNA。值得注意的是，与 Hh 信号通路相关的 DEG 与 26 个 DElncRNA 相关。后来的研究表明 DElncRNA 与生物过程和途径之间存在有趣的联系，包括耐药性、免疫系统控制和致癌特征。与 Hh 信号通路相关的 DEG 具有与已知参与 ALL 的 miRNA 相同的 miRNA，包括 miR-155-5p 和 miR-211，凸显了这种疾病中调控环境的复杂性。Hh 信号通路之间的复杂联系这项研究已揭示了 ALL 中的 、lncRNA 和 miRNA，表明与 Hh 信号通路基因相关的 DElncRNA 有可能作为 ALL 的治疗靶点和诊断生物标志物。© 2024。作者获得 Springer 的独家许可-德国 Verlag GmbH，隶属于施普林格自然集团。

Acute lymphoblastic leukemia (ALL) is a common hematologic cancer with unique incidence and prognosis patterns in people of all ages. Recent molecular biology advances have illuminated ALL's complex molecular pathways, notably the Hedgehog (Hh) signaling system and non-coding RNAs (ncRNAs). This work aimed to unravel the molecular complexities of the link between Hh signaling and ALL by concentrating on long non-coding RNAs (lncRNAs) and their interactions with significant Hh pathway genes.To analyze differentially expressed lncRNAs and genes in ALL, microarray data from the Gene Expression Omnibus (GEO) was reanalyzed using a systems biology approach. Hh signaling pathway-related genes were identified and their relationship with differentially expressed long non-coding RNAs (DElncRNAs) was analyzed using Pearson's correlation analysis. A regulatory network was built by identifying miRNAs that target Hh signaling pathway-related mRNAs.193 DEGs and 226 DElncRNAs were found between ALL and normal bone marrow samples. Notably, DEGs associated with the Hh signaling pathway were correlated to 26 DElncRNAs. Later studies showed interesting links between DElncRNAs and biological processes and pathways, including drug resistance, immune system control, and carcinogenic characteristics. DEGs associated with the Hh signaling pathway have miRNAs in common with miRNAs already known to be involved in ALL, including miR-155-5p, and miR-211, highlighting the complexity of the regulatory landscape in this disease.The complex connections between Hh signaling, lncRNAs, and miRNAs in ALL have been unveiled in this study, indicating that DElncRNAs linked to Hh signaling pathway genes could potentially serve as therapeutic targets and diagnostic biomarkers for ALL.© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.