m6A 表观转录组动力学的新见解：混合序列识别 m6A 写入器 METTL3/14 的新 mRNA。
New insights into the dynamics of m6A epitranscriptome: hybrid-seq identifies novel mRNAs of the m6A writers METTL3/14.
发表日期：2024 Sep 03
作者：
Konstantina Athanasopoulou, Panagiotis G Adamopoulos, Andreas Scorilas
来源：
Epigenetics & Chromatin
摘要：
背景：N6-甲基腺苷 (m6A) 是一种常见的 mRNA 修饰，受甲基转移酶（包括 METTL3 和 METTL14）动态调节。 材料
Background: N6-methyladenosine (m6A), a prevalent mRNA modification, is dynamically regulated by methyltransferases, including METTL3 and METTL14.Materials & methods: In the current study, we employed a custom hybrid-seq method to identify novel METTL3/14 transcripts, explore their protein-coding capacities and predict the putative role of the METTL isoforms.Results: Demultiplexing of the hybrid-seq barcoded datasets unraveled the expression patterns of the newly identified mRNAs in major malignancies as well as in non-malignant cells, providing a deeper understanding of the methylation pathways. Open reading frame query revealed novel METTL3/14 isoforms, broadening our perspective for the structural diversity within METTL family.Conclusion: Our findings offer significant insights into the intricate transcriptional landscape of METTL3/14, shedding light on the regulatory mechanisms underlying methylation in mRNAs.