Hsa_circYARS 与 miR-29a-3p 相互作用，上调 IREB2 并促进喉鳞状细胞癌进展。
Hsa_circYARS interacts with miR-29a-3p to up-regulate IREB2 and promote laryngeal squamous cell carcinoma progression.
发表日期：2024 Sep 03
作者：
Zizhao Guo, Yuxia Zhao, Naicai Guo, Meng Xu, Xiaolei Wang
来源：
Cellular & Molecular Immunology
摘要：
本研究旨在探讨circYARS在喉鳞状细胞癌（LSCC）中的致癌能力，并揭示其作为竞争性内源RNA的潜在机制。通过RT-qPCR检测LSCC中差异表达的circRNA和mRNA。进行双荧光素酶报告基因测定和 RIP 来测试 circYARS、miR-29a-3p 和 IREB2 之间的相互作用。通过CCK-8、流式细胞术、集落形成实验、Transwell、Western blot和异种移植小鼠模型研究这些分子的功能作用。在LSCC组织和细胞系中，circYARS和IREB2水平增强，而miR-29a-3p水平被降低了。耗尽 circYARS 通过促进 miR-29a-3p 表达导致 IREB2 减少。由于miR-29a-3p增强或circYARS沉默，癌细胞的增殖、迁移和侵袭受到抑制，并刺激细胞凋亡。circYARS通过miR-29a-3p/IREB2参与LSCC的致瘤和进展。轴，为 LSCC 的治疗干预提供策略和目标。© 2024。作者。
This study was to investigate the carcinogenic capacity of circYARS in laryngeal squamous cell carcinoma (LSCC) and to reveal its potential mechanism as a competitive endogenous RNA.The differentially expressed circRNA and mRNA in LSCC were detected by RT-qPCR. Dual luciferase reporter assay and RIP were conducted to test the interaction between circYARS, miR-29a-3p, and IREB2. The functional effects of these molecules were investigated by CCK-8, flow cytometry, colony formation assay, Transwell, Western blot, and xenotransplantation mouse models.In LSCC tissues and cell lines, circYARS and IREB2 levels were enhanced, while miR-29a-3p level was lowered. Depleting circYARS led to decreased IREB2 by promoting miR-29a-3p expression. As a result of miR-29a-3p enhancement or circYARS silence, the proliferative, migratory, and invasion of cancer cells were suppressed and apoptosis was stimulated.circYARS is involved in the tumorigenicity and progression of LSCC through the miR-29a-3p/IREB2 axis, providing strategies and targets for therapeutic intervention of LSCC.© 2024. The Author(s).