这项研究提出了一种新颖的、环境可持续的方法来合成氧化石墨烯（GO）片，该氧化石墨烯片均匀地装饰有尺寸为 4 至 34 nm 的银纳米颗粒（Ag NP）。 AgNO3 的还原是通过使用来自黄瓜果实的提取物来实现的，该提取物充当双功能稳定剂和还原剂。胼胝属于葫芦科，原产于印度、南美洲、泰国、非洲和埃及等地区，因其丰富的营养和药用价值而闻名，包括抗氧化、抗糖尿病、抗癌和抗炎特性。在本研究中，我们首次探索利用愈伤组织提取物合成银纳米粒子，采用绿色合成方法生产GO-Ag纳米复合材料。综合表征技术证实了合成纳米复合材料的结构完整性和质量。使用纸片扩散法评估不同剂量的绿色合成的Ag装饰的GO纳米复合材料对枯草芽孢杆菌（革兰氏阳性）和大肠杆菌（革兰氏阴性）细菌的抗菌功效。纳米复合材料对两种细菌菌株均表现出剂量依赖性抗菌活性，并且对革兰氏阴性菌的作用显着增强。这些发现强调了胼胝作为可持续制备 GO-Ag 纳米复合材料的潜力，该复合材料具有增强的抗菌性能，适用于各种生物医学和环境应用。研究亮点：这项工作提出了一种简单、环保且经济高效的绿色合成方法，用于在 GO 片上装饰均匀的小（4-34 nm）球形银纳米颗粒。 Ag NPs 是通过使用黄瓜果实提取物作为稳定剂和还原剂还原 AgNO3 来生产的。纳米复合材料对革兰氏阳性菌和革兰氏阴性菌均表现出剂量依赖性抗菌活性，但对革兰氏阴性菌的抗菌效果更高。使用胼胝等草本植物/水果通过绿色途径合成这些纳米复合材料将使医疗行业受益。© 2024 Wiley periodicals LLC。

This study presents a novel, environmentally sustainable method for the synthesis of graphene oxide (GO) sheets decorated uniformly with silver nanoparticles (Ag NPs) ranging in size from 4 to 34 nm. The reduction of AgNO3 is achieved using an extract derived from Cucumis callosus fruit, which serves as a dual-function stabilizing and reducing agent. Cucumis callosus, belonging to the Cucurbitaceae family and native to regions such as India, South America, Thailand, Africa, and Egypt, is recognized for its substantial nutritional and medicinal value, encompassing antioxidant, antidiabetic, anticancer, and anti-inflammatory properties. In this study, we explore the utilization of Cucumis callosus extract for the first time in synthesizing Ag NPs, employing a green synthesis approach to produce GO-Ag nanocomposites. Comprehensive characterization techniques confirm the structural integrity and quality of the synthesized nanocomposites. The antibacterial efficacy of the green-synthesized Ag-decorated GO nanocomposites was evaluated using the disk diffusion method against Bacillus subtilis (Gram-positive) and Escherichia coli (Gram-negative) bacteria at varying dosages. The nanocomposites demonstrated dose-dependent antibacterial activity against both bacterial strains, with a notably heightened effect observed against Gram-negative bacteria. These findings underscore the potential of Cucumis callosus as a promising candidate for the sustainable preparation of GO-Ag nanocomposites with enhanced antibacterial properties, suitable for various biomedical and environmental applications. RESEARCH HIGHLIGHTS: This work presents a simple, environmentally free, and cost-effective green synthesis method to decorate uniformly small (4-34 nm) spherical Ag NPs on the GO sheets. Ag NPs were produced by reducing AgNO3 using Cucumis callosus fruit extract as a stabilizing and reducing agent. The nanocomposites show dosage-dependent antibacterial activities against both Gram-positive and Gram-negative bacteria, but the antibacterial effect is higher against the Gram-negative bacteria. Synthesis of these nanocomposites via the green route using an herbal plant/fruit like Cucumis callosus will benefit the medical industry.© 2024 Wiley Periodicals LLC.