长非编码 RNA CRNDE、LINC00957 和 AC072061.1 作为多形性胶质母细胞瘤有前景的诊断和预后生物标志物。
Long Non-Coding RNA CRNDE, LINC00957, and AC072061.1 as a Promising Diagnostic and Prognostic Biomarker in Glioblastoma Multiforme.
发表日期：2024 Sep
作者：
Arash Poursheikhani, Meysam Mosallaei, Mohammad Foad Heidari, Mohsen Rajaeinejad, Mohsen Chamanara, Mojtaba Yousefi Zoshk, Peyman Aslani, Ebrahim Hazrati, Mojgan Mohammadimehr, Javad Behroozi
来源：
Parasites & Vectors
摘要：
多形性胶质母细胞瘤（GBM）是最具侵袭性的脑癌类型之一。 LncRNA 可以被认为是 GBM 中新的预后和诊断生物标志物。本研究全面探讨了TCGA数据库中lncRNA与mRNA的相互作用，提出了一种具有良好诊断和预后价值的新型有前景的生物标志物。RNA-seq的公开数据和相关临床数据均从TCGA数据库下载。在R中进行差异表达分析。利用GO和KEGG信号通路进行富集。 STRING 数据库用于 PPI 分析。 CE-网络是利用STAR数据库构建的。 Kaplan-Meier生存分析和ROC曲线分析表明生物标志物的诊断和预后价值。差异表达数据表明4428个mRNA在GBM中差异表达。 GO和KEGG通路分析表明差异表达的mRNA在关键生物过程中富集。 PPI 显示 WEE1、BARD1 和 CDK6 是重要的 PPI 中心。 ceRNA 网络数据证明了关键的 lncRNA。数据显示，lncRNA CRNDE、LINC00957、AC072061.1、AC068888.1 和 DBH-AS1 是 GBM 患者的潜在诊断预后生物标志物。总之，我们证明了 lncRNA 和 mRNA 相互作用，并提到了调节网络，被认为是一种治疗选择在GBM中。此外，我们还为患者提出了潜在的诊断和预后生物标志物。版权所有© 2024 Poursheikhani 等人。由德黑兰医科大学出版。
Glioblastoma multiforme (GBM) is one of the most invasive types of brain cancer. LncRNAs can be considered a new prognostic and diagnostic biomarker in GBM. This study comprehensively explored the interaction of lncRNAs with mRNAs in the TCGA database and proposed a novel promising biomarker with favorable diagnostic and prognostic values.The public data of RNA-seq and related clinical data were downloaded from the TCGA database. Differential expression analysis was conducted in R. GO and KEGG signaling pathways were used for enrichment. The STRING database was used for PPI analysis. CE-network was constructed by STAR database. Kaplan-Meier survival analysis and ROC curve analysis to indicate the biomarkers' diagnostic and prognostic values.Differentially expressed data illustrated that 4428 mRNAs were differentially expressed in GBM. The GO and KEGG pathway analysis showed that the differentially expressed mRNAs were enriched in critical biological processes. The PPI showed that WEE1, BARD1, and CDK6 were the important PPI hubs. The ceRNA network data demonstrated critical lncRNAs. The data revealed that the lncRNA CRNDE, LINC00957, AC072061.1, AC068888.1, and DBH-AS1 are potential diagnostic prognostic biomarkers in the GBM patients.Altogether, we demonstrated lncRNA, and mRNA interaction and mentioned regulatory networks, considered a therapeutic option in GBM. In addition, we proposed potential diagnostic and prognostic biomarkers for the patients.Copyright© 2024 Poursheikhani et al. Published by Tehran University of Medical Sciences.