前列腺癌（PCa）是男性泌尿生殖系统最常见的恶性肿瘤。了解PCa的分子机制及其预后标志物将有助于选择更好的治疗方案。探讨LINC01518在PCa发生发展中的作用及其预后价值，了解其具体的调控机制。LINC01518、hsa-的水平通过 RT-qPCR 检测 miR-320a 和 CNKSR2 mRNA。构建 ROC 曲线来评估 LINC01518 对 PCa 的预后价值。 LINC01518敲除后，通过CCK-8、Transwell试验和凋亡标志物检测证明了LINC01518对PCa细胞功能的影响。通过构建荧光素酶载体检测hsa-miR-320a与LINC01518或CNKSR2 mRNA之间的相互作用。LINC01518在PCa细胞和肿瘤组织中异常表达，敲低它会抑制PCa细胞的生长。 LINC01518 预测 PCa 患者的去势抵抗性前列腺癌 (CRPC) 结局，AUC 为 0.803，敏感性和特异性分别为 75.4% 和 74.6%。 Hsa-miR-320a 模拟转染 WT-LINC01518/CNKSR2 质粒的 PCa 细胞中荧光素酶活性的降低。敲低LINC01518会降低CNKSR2水平，这种调节作用随着hsa-miR-320a的抑制而消失。高水平的LINC01518预示PCa患者预后不良，并通过竞争性结合hsa-miR-320a促进CNKSR2表达，促进PCa进展。 PCa.© 2024。作者。

Prostate cancer (PCa) is the most common malignancy of the male genitourinary system. Understanding the molecular mechanism of PCa and its prognostic markers will assist in the selection of better treatment.To explore the role of LINC01518 in the development of PCa and its prognostic value, and to understand its specific regulatory mechanism.The levels of LINC01518, hsa-miR-320a, and CNKSR2 mRNA were detected by RT-qPCR. ROC curve was constructed to evaluate the prognostic value of LINC01518 in PCa. The effects of LINC01518 on the functions of PCa cells were demonstrated by CCK-8, Transwell test, and the detection of apoptotic markers, after LINC01518 knockdown. The interaction between hsa-miR-320a and LINC01518 or CNKSR2 mRNA was examined by constructing luciferase vectors.LINC01518 was abnormally expressed in PCa cells and tumor tissues, and knockdown of it inhibited the growth of PCa cells. LINC01518 predicted castration-resistant prostate cancer (CRPC) outcomes in PCa patients with AUC of 0.803, sensitivity and specificity of 75.4% and 74.6%, respectively. Hsa-miR-320a mimics reduced luciferase activity in PCa cells transfected with WT-LINC01518/CNKSR2 plasmids. Knocking down LINC01518 reduced CNKSR2 level, and this regulatory effect disappeared with the inhibition of hsa-miR-320a.High levels of LINC01518 predicted poor prognosis in PCa patients and promoted CNKSR2 expression by competitively binding hsa-miR-320a, contributing to the progression of PCa.© 2024. The Author(s).