海参既是用途广泛的海洋生物，也是一种具有多种药用功效的亚洲海洋食品。我们评估了来自黑海参体壁的一些主要纯化全甾烷型皂苷的抗过敏潜力。分离出六种皂苷化合物：海参素 B (1)、海参素 A (2)、24-脱氢海胆苷 A (3)、去海参素 A1 (4)、去海参素 A (5) 和去氢海胆苷 A (6)。基于光谱方法并通过与文献比较来鉴定结构。评估了每种化合物对β-氨基己糖苷酶释放的抑制活性。在六种化合物中，与阳性对照槲皮素相比，海参素 B (1) 在所有测试浓度下均以剂量依赖性方式显示出最强的脱颗粒抑制作用。我们还观察到海参素 B (1) 能够减轻炎症介质白细胞介素 (IL)-6、IL-13 和肿瘤坏死因子-α (TNF-α)。 Holothurin B (1) 还通过抑制肌醇-1,4,5-三磷酸受体 (IP3R) mRNA 的表达来抑制钙离子载体 A23187 刺激的 Ca2 内流。这些结果表明，(i) 海参素 B (1) 在有效浓度下具有良好的抗过敏活性，且无细胞毒性，并且 (ii) 该化合物可能是治疗过敏性疾病和相关炎症的先导化合物。我们还对测试化合物进行了分子对接研究，将它们的结合模式和对 IP3R 的亲和力与体外结果关联起来。结果得出结论，全甾烷型皂苷可用作抗过敏剂，这可能归因于其全甾烷基团。在线版本包含可在 10.1007/s10616-024-00649-8 获取的补充材料。© 作者(s) ），获得 Springer Nature B.V. 2024 的独家许可。Springer Nature 或其许可方（例如协会或其他合作伙伴）根据与作者或其他权利持有人的出版协议拥有本文的专有权；作者对本文已接受的手稿版本的自行存档仅受此类出版协议和适用法律的条款的约束。

Sea cucumbers are both versatile marine organisms and an Asian marine food known to have several medicinal effects. We evaluated the anti-allergic potential of some major purified holostane-type saponins from the body wall of the black sea cucumber, Holothuria atra. Six saponin compounds were isolated, holothurin B (1), holothurin A (2), 24-dehydro echinoside A (3), desholothurin A1 (4), desholothurin A (5), and des 24-dehydro echinoside A (6). The structures were identified based on spectroscopic methods and by comparison with the literature. Each compound's inhibitory activity toward the release of β-hexosaminidase was evaluated. Among the six compounds, holothurin B (1) showed the strongest inhibition of the degranulation at all tested concentrations in a dose-dependent manner, compared to the positive control, quercetin. We also observed that holothurin B (1) was able to alleviate the inflammatory mediators interleukin (IL)-6, IL-13, and tumor necrosis factor-alpha (TNF-α). Holothurin B (1) also inhibited the Ca2+ influx stimulated by the calcium ionophore A23187, by suppressing the expression of inositol-1,4,5-triphosphate receptor (IP3R) mRNA. These results suggest that (i) holothurin B (1) has good anti-allergy activity without cytotoxicity at effective concentrations, and (ii) this compound could be a lead compound for the treatment of allergic diseases and associated inflammation. We also performed a molecular docking study for the tested compounds to correlate their binding modes and affinity for the IP3R with the in vitro results. The results concluded that the holostane-type saponins could be used as anti-allergy agents, which may be attributed to their holostane group.The online version contains supplementary material available at 10.1007/s10616-024-00649-8.© The Author(s), under exclusive licence to Springer Nature B.V. 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.